肝纤维化大鼠肝组织Na^+/Ca^(2+)泵mRNA表达  

Expression of Na^+/Ca^(2+) exchanger in liver tissues of rats with hepatic fibrosis

作　　者：曾维政[1] 吴晓玲[2] 陈晓斌[1] 蒋明德[1] 邓桂英[3] 熊碧君[1] 秦建平[1] 罗克枢[4] 

机构地区：[1]成都军区总医院消化内科,四川成都610083 [2]重庆医科大学附一医院消化内科,重庆400016 [3]成都市第六人民医院胃镜室,四川成都610051 [4]成都军区总医院病理科,四川成都610083

出　　处：《第四军医大学学报》2005年第10期919-922,共4页Journal of the Fourth Military Medical University

基　　金：成都军区"十五"医药卫生科研基金(01A009)

摘　　要：目的: 了解肝纤维化大鼠肝组织Na+ /Ca2+泵(Na+ /Ca2+ exchanger,NCX)mRNA表达状况,探讨其与肝纤维化程度的关系及意义.方法: CCl4 sc法诱导大鼠肝纤维化,观察肝组织NCX及α1(Ⅰ)mRNA表达水平,检测血清HA,CⅣ,PCⅢ,血清肝纤维化相关酶(βNAG,MAO,ALD)、肝组织羟脯氨酸(hydroxyproline,Hyp)含量及肝组织病理学变化,分析其相关性与临床意义.结果: 20只正常大鼠肝组织NCXmRNA表达除1只弱阳性外,其余均为阴性,半定量积分仅为(0.10±0. 00);而30只模型组大鼠全部呈阳性,半定量积分显著增加(P< 0 .05 ).与正常组比较,模型组大鼠血清HA[ (455. 79±113 .55)vs(112 .41±45. 62)μg/L],CⅣ, [ (159. 62±29 64)vs(35 69±9 68)μg/L],PCⅢ[ (265 .54±98. 21)vs(89 .99±10. 85 ) μg/L]明显增高(均为P<0 01 );βNAG,MAO,ALD活性显著增加(分别增加2. 20, 2. 14, 1 .57倍,P<0 01),模型组大鼠NCXmRNA表达与α1(Ⅰ)mRNA表达水平、肝组织Hyp含量、血清HA,CⅣ,PCⅢ水平、肝组织纤维化积分均呈正相关,相关系数r分别为+0. 48, +0. 64, +0 .52,+0 49, +0 .56, +0 .60(均为P<0 05).结论: 肝纤维化大鼠肝组织NCXmRNA表达明显增强,与胶原代谢水平及肝脏组织病理学变化呈正相关,有望作为判断肝纤维化的有用指标,值得进一步?AIM: To observe the expression level of Na+/Ca 2+ exchanger mRNA in rats with hepatic fibrosis induced by CCl_4,to investigate its relationship with collagen metabolism and to explore its diagnosis value for hepatic fibrosis.METHODS: Hepatic fibrosis rat model was induced by subcutaneous injection of CCl_4.The expressions of Na+/Ca 2+ exchanger mRNA and α1(Ⅰ) procollagen mRNA were determined by RT-PCR in situ.The content of hydroxyproline in liver tissue was detected with Jamall's method and the concentration of HA,CⅣ and PCⅢ in serum was measured with RIA method.The deposition of collagen in the liver tissue was observed with HE,VG and Masson staining. RESULTS: The expressions of Na+/Ca 2+ exchanger mRNA and α1(Ⅰ) procollagen mRNA in the liver of rats with hepatic fibrosis increased obviously (semiquantitative score 2.45 vs 0.10,P<0.05).Also significantly increased were the content of hydroxyproline in liver tissue and the concentration of HA [(455.79±113.55) vs (112.41±45.62) μg/L,P<0.01],CⅣ [(159.62±29.64) vs (35.69±9.68) μg/L,P<0.01] and PCⅢ [(265.54±98.21) vs (89.99±10.85) μg/L,P<0.01] in serum of rats with liver fibrosis.The change of these parameters was parallel to the pathological damage of the liver (r = +0.48,+0.64,+0.52,+0.49,+0.56 and +0.60 respectively,P<0.05).CONCLUSION: Na+/Ca 2+ exchanger can be a useful index for the diagnosis of hepatic fibrosis,and further study is needed.

关 键 词：大鼠 肝硬化 钠钙交换蛋白 

分 类 号：R575.2[医药卫生—消化系统]