输液用蛋黄磷脂中溶血性组分的薄层色谱分析  

TLC Analysis of Hemolytic Components in Egg Yolk Phospholipids

作　　者：安永祥[1] 宋航[1] 黄明刚[1] 付超[1] 

机构地区：[1]四川大学化工学院制药与生物工程系,成都610065

出　　处：《药物分析杂志》2005年第5期562-564,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立输液用蛋黄磷脂中溶血性组分 LPC,LPE 的薄层色谱分析方法。方法:选择并优化展开剂,对溶血性组分LPC,LPE 进行定性、定量分析。结果:展开剂为氯仿-无水乙醇-三乙胺-水(10∶10∶5∶3)时,分离效果良好,得到7个磷脂组分的定性结果。LPC 在0.25-2.5μg线性度较好,r=0.9901,LPE 的质量情况得到分析。结论:本法简便,快速,可作为静脉输液用磷脂质量监控的有效方法。Objective:A TLC method has been developed to analyze hemolytic components lysophosphatidyl choline (LPC)and lysophosphatidyl ethanolamine(LPE)in egg yolk phospholipids used for transfusion.Methods:Selected and optimized mobile phase.LPC and LPE in phospholipids were identified by TLC,and LPC was determined by TLC-scanning.Results:The best mobile phase was chloroform-ethanol-triethylamine-water(10:10:5:3). Seven components in egg yolk phospholipids were separated by TLC.The linear concentration range of LPC by TLC was 0.25-2.5 μg with a correlation coefficient of 0.9901.Conclusion:This method is accurate,simple,and can be used for the quality control of egg yolk phospholipids.

关 键 词：蛋黄磷脂 薄层色谱分析 溶血性 输液用 色谱分析方法 LPC 定量分析 无水乙醇 分离效果 磷脂组分 有效方法 质量监控 展开剂 三乙胺 线性度 5μg 定性 

分 类 号：R977.6[医药卫生—药品] R979.19[医药卫生—药学]