Fmoc(9-芴甲氧羰基)法固相合成胸腺五肽  

The solid-phase peptide synthesis(SPPS) of thymopentin(TP5) by Fmoc strategy

作　　者：欧阳嘉[1] 李鑫[2] 潘宗敏[1] 沈树宝[2] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210009 [2]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《南京工业大学学报（自然科学版）》2005年第3期61-64,共4页Journal of Nanjing Tech University(Natural Science Edition)

摘　　要：采用对碱敏感的Fmoc法α-氨基保护策略固相合成了胸腺五肽,并讨论了胸腺五肽不同固相载体、不同活化试剂对胸腺五肽固相合成的影响。试验结果表明Fmoc合成策略中各步的缩合率均在90%以上,同时对产品进行色谱分析,纯度达到83%。Using the base-labile Fmoc group to protect α-NH 2 of amino acid, the thymopentin(TP5) has been synthesized by a solid-phase method. Then, different linkers and active reagents were investigated to optimise the synthesis of TP5 by SPPS. The experimental results showed that the ratio of the coupling cycle of each amino acid was over 90%. After cleavage of peptide resin, the purity of rude TP5 was about 83% by HPLC analysis.

关 键 词：胸腺五肽 固相合成 甲氧羰基 FMOC 保护策略 Α-氨基 固相载体 试验结果 色谱分析 芴 试剂 缩合 

分 类 号：TQ464.7[化学工程—制药化工] TB383[一般工业技术—材料科学与工程]