杂色曲霉素对体外小鼠腹腔巨噬细胞白细胞介素12表达与分泌的影响  

Effect of sterigmatocystin on interleukin-12 expression and secretion of murine peritoneal macrophage cells in vitro

作　　者：邢凌霄[1] 张祥宏[1] 尹桂然[2] 李月红[1] 王俊灵[1] 严霞[1] 王凤荣[1] 

机构地区：[1]河北医科大学基础所病理室,河北石家庄050017 [2]河北医科大学第二医院妇产科研究室,河北石家庄050000

出　　处：《中国药理学与毒理学杂志》2005年第3期205-208,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家科技部基础研究重大项目前期研究专项资助项目 (2 0 0 1CCC0 0 5 0 0 ) ;河北省自然科学基金重点资助项目 (30 135 0 )~~

摘　　要：目的　探讨杂色曲霉素(ST)对巨噬细胞免疫功能的影响。方法　分别采用半定量逆转录聚合酶链反应及酶联免疫吸附法,研究0 .12 5 ,0 .2 5 ,0 .5 ,1.0和2 .0mg·L- 1ST预处理对小鼠腹腔巨噬细胞白细胞介素(IL) 12mRNA表达及其蛋白分泌的影响。结果　在0 .12 5～1.0mg·L- 1范围内,ST处理对小鼠腹腔巨噬细胞IL 12p4 0和IL 12p35mRNA的表达均有一定的抑制作用。但在蛋白水平,对IL 12分泌的影响不明显。当ST浓度达到2 .0mg·L- 1时,可明显抑制IL 12p4 0mRNA的表达及IL 12的分泌。结论　ST可抑制巨噬细胞IL 12的表达或分泌,提示ST可能在一定程度上对机体的细胞免疫功能产生不利影响。AIM To explore the effect of sterigmatocystin (ST) on immunologic function of macrophages. METHODS The secretion and expression of interleukin(IL)-12 of murine peritoneal macrophages after ST pretreatment at different concentrations(0.125,0.25,0.5,1.0 and 2.0 mg ·L -1 ) were studied with ELISA and semi-quantitative RT-PCR method, respectively. RESULTS In the concentration range 0.125-1.0 mg·L -1 , ST could inhibit the expression of IL-12 p40 and IL-12 p35 at mRNA level, but no effect on the secretion of IL-12 protein was found. ST at 2.0 mg·L -1 could significantly inhibit the expression of IL-12 p40 mRNA and the secretion of IL-12 protein. CONCLUSION ST could inhibit the expression or secretion of IL-12 of murine peritoneal macrophages.

关 键 词：杂色曲霉素 白细胞介素12 巨噬细胞 腹膜 逆转录聚合酶链反应 

分 类 号：R99[医药卫生—毒理学]