HL-60细胞ATRA诱导后MMP-9/TIMP-1表达的变化  

The expression of the matrix metalloproteinas-9 in HL-60 cells after treatment with all-trans-retinoic acid

作　　者：王俊伟[1] 张玉昆[1] 唐天华[1] 任海全[1] 张伟[1] 姜国胜[1] 

机构地区：[1]山东省医学科学院基础医学研究所,山东省医药卫生肿瘤免疫与中药免疫重点实验室,山东省医学科学院血液肿瘤防治研究中心,济南250062

出　　处：《现代免疫学》2005年第3期227-230,共4页Current Immunology

基　　金：教育部留学人员科研启动基金资助项目(2004-527)山东省优秀中青年科学家基金项目(03BS033)山东省自然科学基金重点项目(Z2004C08)国家科技部中瑞政府间科技合作项目(AM15B:18)

摘　　要：为了检测HL-60细胞经过全反式维甲酸(ATRA)诱导后基质金属蛋白酶(MMP-9、MMP-2)及金属蛋白酶组织抑制剂1(TIMP-1)表达变化,并探讨其变化的意义。采用瑞氏染色观察细胞形态,WST1实验测定细胞增殖变化,NBT还原实验测定细胞分化状态,RT-PCR方法检测MMP-9、MMP-2、TIMP-1、VEGF的mRNA的表达。结果显示,HL-60细胞经ATRA作用24h后,随着细胞增殖降低与分化的发生,MMP-9mRNA表达增加,TIMP-1mRNA和VEGF表达降低,MMP-2mRNA未见明显表达。ATRA诱导HL-60细胞分化后MMP-9表达增加,而TIMP-1表达降低,MMP-9不促进HL-60细胞表达VEGF。To investigate the expressions of matrix metalloproteinase-9(MMP-9) and MMP-2 as well as the MMP tissue inhibitor-1 (TIMP-1) in leukemia cell HL-60 cells after treatment with all-trans-retinoic acid (ATRA) and to explore the implication of this investigation, the changes of cell morphology were observed by Wright staining, the alteration in the cell proliferation was determined by WST1 experiment and the NBT reduction assay was used to detect the differentiation condition of cells. The expressions of mRNA of MMP-9, MMP-2, VEGF and TIMP-1 in HL-60 cells after exposure to ATRA were assayed by semi-quantitative RT-PCR. It was found that ATRA could up-regulate the mRNA expression of MMP-9, but down-regulate those of TIMP-1 or VEGF. The mRNA expression of MMP-2 in HL-60 cells could not be demonstrated. It is concluded that ATRA induces an increase of MMP-9 expression after differentiation of HL-60 cells without up-regulation of VEGF expression.

关 键 词：基质金属蛋白酶 全反式维甲酸 

分 类 号：R733.7[医药卫生—肿瘤]