高盐对SD大鼠肾脏骨调素mRNA表达的影响  

Effect of high salt diet on expression of osteopontin mRNA in

作　　者：郑勇[1] 刘治全[1] 叶涛[1] 何岚[2] 王毅[2] 方园[1] 李建宁[2] 刘萍[2] 叶枫[2] 

机构地区：[1]西安交通大学第一医院心内科,陕西西安710061 [2]西安交通大学第一医院内分泌科,陕西西安710061

出　　处：《第一军医大学学报》2005年第5期595-597,共3页Journal of First Military Medical University

摘　　要：目的观察高盐饮食对大鼠肾脏骨调素mRNA及其蛋白表达的影响,旨在研究高盐对大鼠肾脏的损伤作用。方法选取10周龄正常盐饮食雄性Sprague-Dawley大鼠48只,随机分为高盐(4%NaCl)或正常盐(0.6%NaCl)饮食组,每组24只。每周测量大鼠鼠尾血压、体质量。于第4、8、12周末每组各处死6只大鼠,测量大鼠肾质量。采用免疫组化方法测定大鼠肾脏OPN表达;荧光定量PCR方法测定大鼠肾脏骨调素mRNA表达。结果两组大鼠血压、体质量及肾质量/体质量比无明显差异(P<0.05),12周后高盐饮食大鼠肾脏骨调素mRNA(0.27±0.16vs0.15±0.13,P<0.05)及其蛋白表达明显升高(0.78±0.15vs0.61±0.11,P<0.01),与正常盐饮食大鼠比较有显著统计学差异。结论高盐饮食摄入增加大鼠肾脏骨调素mRNA及其蛋白表达,表明高盐对大鼠肾脏有一定损伤作用,并且这种损伤作用不依赖于大鼠血压升高。Objective To investigate the effect of high-salt diet on the expression of osteopontin (OPN) mRNA and its protein in Sperague-Dawley rat kidney. Methods Forty-eight male SD rats aged 10 weeks receiving normal salt diet were enrolled in study, and were divided into two groups and fed with high salt diet (4%NaCl) or normal salt diet (0.6%NaCl) for 12 weeks respectively, with 24 rats in each group. Tail systolic blood pressure and bodyweight were measured in all rats every week. At the end of 4th, 8th and 12th week, 6 rats in each group were sacrificed for detection of the expression of OPN mRNA and its protein in the kidney with quantitatine real-time (QRT)-PCR and immunohistochemistry, respectively. Results No significant difference was found in blood pressure, bodyweight and kidney weight/bodyweight between high-salt diet group and normal salt diet group (P>0.05). The expression of OPN mRNA (0.27±0.16 vs 0.15±0.13, P<0.05) and its protein (0.78±0.15 vs 0.61±0.11, P<0.01) in SD rat kidney with high-salt diet were up-regulated at the end of 12th week compared with the rats with normal salt diet. Conclusions High-salt diet increase the expression of OPN mRNA and its protein in SD rat kidney. Renal injury induced by high salt intake might be independent of blood pressure change.

关 键 词：高盐饮食 血压 肾脏损伤 骨调素 

分 类 号：R363[医药卫生—病理学]