bcl-2反义核酸提高γ线对恶性淋巴瘤细胞凋亡的作用  

Enhancement of bcl-2 antisense oligodeoxynucleotide on γ-ray induced-apoptos is in malignant lymphoma cells

作　　者：聂林[1] 张洹[2] 李萍[1] 郭良君[1] 王奕鸣[1] 庄承海[1] 

机构地区：[1]暨南大学附属第一医院肿瘤科,广东广州510630 [2]暨南大学医学院血液病研究所

出　　处：《中华放射肿瘤学杂志》2005年第2期112-115,共4页Chinese Journal of Radiation Oncology

基　　金：广州市科技计划重点基金 (2 0 0 1 Z 0 3 7 0 1 1) ;广东省自然科学重点基金 (0 2 1195 ) ;广东省医学科学技术研究基金资助项目 (B2 0 0 2 0 69)

摘　　要：目的　研究bcl -2反义核酸能否增加60 Coγ线对恶性淋巴瘤细胞凋亡的作用。方法　采用细胞染色方法观察细胞凋亡的形态 ,原位末端标记法检测凋亡细胞 ,流式细胞仪检测细胞的DNA含量和bcl 2蛋白的表达。结果　 1～ 8Gyγ线和 10～ 4 0 μmol/Lbcl- 2基因反义核酸均能抑制B淋巴瘤细胞株Raji细胞生长 ,诱导细胞凋亡。流式细胞仪检测亚G1期细胞增多 ,bcl- 2蛋白表达降低 ,呈现时间剂量依赖效应 ,联合应用比单独应用抑制作用显著。结论　bcl- 2反义核酸能够增加60 Coγ线对恶性淋巴瘤细胞凋亡的作用。Objective To investigate whether bcl-2 antisense oligodeoxy nucleotid e (ASODN) could enhance 60Co γ-ray induced-apoptosis in mal ignant lymphoma cells . Methods Cell apoptosis situation, cellular DNA contents and bcl-2 protein exp ression were determined by cell stain, TdT-mediated dUTP Nick-end Labeling (TU NE L) and flow cytometry. Results One to 8?Gy γ-ray and 10-40?μmol/L bcl-2 ASODN could inhibit Raji cell growth and induce cell apoptosis. Assay of flow cytometr y showed that the number of Sub-G 1 cells was significantly increased and Bcl -2 p rotein expression in Raji cells was significantly downregulated, which showed ti me- and dose-dependent manners. The effects were more pronounced when γ-ray and bcl-2 ASODN were used together rather than either alone. Conclusion Bcl-2 antis ense oligodeoxynucleotide could enhance 60Co γ-ray induced -apoptosis in malignant lymphoma cells.

关 键 词：BCL-2反义核酸 Γ线 恶性淋巴瘤 细胞凋亡 肿瘤细胞 RAJI细胞 

分 类 号：R733.1[医药卫生—肿瘤]