检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:李杰[1] 陈孝平[1] 时昌文[2] 徐宗珍[2]
机构地区:[1]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030 [2]山东省千佛山医院普外中心
出 处:《中华实验外科杂志》2005年第6期680-682,i002,共4页Chinese Journal of Experimental Surgery
摘 要:目的观察环氧合酶(COX)-2在肝癌细胞系(HepG2、HLE、HG116、SMMC7402)中蛋白、mRNA水平的表达,探讨COX-2的选择性抑制剂美洛昔康对肝癌细胞系HepG2的生长抑制作用。方法用逆转录聚合酶链反应(RTPCR)和细胞免疫化学方法研究COX-2在人肝癌细胞系中的表达,并用噻唑蓝(MTT)法和流式细胞仪检测COX2的抑制剂美洛昔康对人肝癌细胞系HepG2的生长抑制作用。结果应用免疫组织化学和RTPCR在4种肝癌细胞系中检测COX-2蛋白及其mRNA均有阳性表达。美洛昔康对肝癌细胞系HepG2有较明显的生长抑制作用(P<0.01),并呈时间、剂量依赖性。时间相同时,50μmol/L浓度组抑制率最高,在1、3、5d的抑制率分别为12.5%、39.1%、56.1%,与5、20μmol/L组比较差异有统计学意义(P<0.05);5μmol/L组与20μmol/L组之间差异无统计学意义(P>0.05)。浓度相同时,第5天抑制率最高,5、20、50μmol/L组抑制率分别可达15.6%、28.1%、56.1%,第5天与第1天差异有统计学意义(P<0.01),结论美洛昔康作为COX-2选择性抑制剂对肝癌细胞有明显的生长抑制作用,为肝癌的防治提供了一个新的实验依据。Objective To investigate the cyclooxygenase -2 (COX-2) expression in hepatocellular carcinoma cell lines and the inhibitory effect on the growth of HepG-2 cell line by selective inhibitor of COX-2,Meloxicam.Methods The COX-2 expression in 4 HCC cell lines was detected by immunohistochemistry and RT-PCR.MTT and flow cytometry were used to observe the effect of Meloxicam on the growth of HepG-2 cell line.Results The COX-2 mRNA and protein expression was detectable in all 4 cell lines by immunohistochemistry and RT-PCR technique respectively.Meloxicam could inhibit the growth of HepG-2 cell line in a time- and dose-dependent manner.The growth inhibiting rate in 50 μmol/L group at first,third,and 5th day was 12.5%, 39.1%, 56.1% respectively.There was an obvious difference between 50 μmol/L group and 5 μmol/L,20 μmol/L groups (P< 0.05);At the same dose,the 5 day's growth inhibiting rate of 5 μmol/L,20 μmol/L,50 μmol/L group was 15.6%, 28.1% and 56.1% respectively,which was higher than that on the first day's (P< 0.01).Conclusion Meloxicam could inhibit the growth of HepG-2 HCC cell line in a time- and dose-dependent manner.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.31