转化生长因子-β1对乳腺癌细胞株增殖和T淋巴细胞免疫的影响  

Effect of transforming growth factor-beta 1 on the proliferation of breast cancer cell lines and T lymphocyte transducted cellular immuno-activity

作　　者：徐茜[1] 刘福安[2] 吴莺[1] 赵赟[1] 吴剑宏[3] 龚建平[3] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉430030 [2]华中科技大学同济医学院 [3]华中科技大学同济医学院附属同济医院普通外科,武汉430030

出　　处：《中华实验外科杂志》2005年第6期695-697,共3页Chinese Journal of Experimental Surgery

基　　金：课题基金项目:国家杰出青年基金资助项目(30025017)

摘　　要：目的检测转化生长因子(TGF)-β1对乳腺癌细胞株增殖能力和T淋巴细胞免疫的影响,探讨TGF-β1在乳腺癌演进过程中的作用。方法噻唑蓝(MTT)比色法检测TGFβ1对乳腺癌细胞株MCF7、MDAMB157、SKBR3和MDAMB453增殖的影响,探求其调控细胞生长的浓度效应和时间效应。酶联免疫吸附试验(ELISA)检测TGFβ1作用于体外培养的外周血单个核细胞(PBMC)前后白细胞介素(IL)2和干扰素(IFN)γ的分泌。结果作用72h内TGFβ1对乳腺癌细胞有增殖抑制作用(P<0.05),不同的浓度(1.0～100.0)μg/L产生的效果不同。96h后效应减弱至消失。在TGF-β1作用下,PBMC培养上清中IL2(296.5±79.4)ng/L和IFNγ水平(665.3±107.0)ng/L明显低于无TGFβ1作用组(644.9±105.5)ng/L和(922.3±184.1)ng/L,(P<0.01)。结论TGFβ1对乳腺癌细胞的增殖有短暂抑制作用,在一定浓度范围内呈剂量依赖性,作用时间有限。TGF-β1能降低T细胞的细胞因子释放,抑制细胞免疫。Objective To explore the fuction of transforming growth factor beta1(TGF-β1) in the progression of breast cancer and T lymphocyte transducted cellular immuno-reaction.Methods The effects of TGF-β1 on the preliferation of breastl cancer cell lines MCF-7,MDA-MB-157,SK-BR-3 and MDA-MB-453 were detected by MTT and the dosage-effect and time-effect relationship between cells and TGF-β1 were analyzed.Using enzyme-linked immumoadsorbent assay (ELISA),the production of IL-2 and IFN-γ in the presence or absence of TGF-β1 was measured.Results TGF-β1 could inhibit the growth of breast cancer cells with different effects at different concentrations only in the first three days.Under the regulation of TGF-β1,the concentration of IL-2 (296.5± 79.4) ng/L and IFN-γ (665.3± 107.0) ng/L in the supernatant of cultured PBMC was significantly lower than in the control group [(644.9± 105.5) ng/L and (922.3± 184.1) ng/L respectively, P< 0.001].Conclusion TGF-β1 can inhibit the proliferation of breast cancer cells in a certain range of concentration and in a very short duration.It can inhibit the production of cytokines to depress the cellular immunization.

关 键 词：转化生长因子-Β1 乳腺癌 肿瘤细胞株 细胞增殖 T淋巴细胞 免疫反应 

分 类 号：R737.9[医药卫生—肿瘤]