单克隆抗体及醋酸纤维薄膜电泳法在肺癌血清学诊断上的应用  

Application of Monoclonal Antibodies to Detection the Patients withLung Cancer by Electrophoresis and Enzyme Staining

作　　者：林星石[1] 张晓平[1] 费丽华[1] 袁玫[1] 李力[1] 李华[1] 邢惠清[1] 刘爱兵[1] 

机构地区：[1]解放军总医院肿瘤单克隆室,北京军区总医院

出　　处：《军医进修学院学报》1994年第2期79-81,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：肺癌是我国最常见的恶性肿瘤之一。为提高肺癌的诊治率，我们对肺癌血清学的诊断方法不断进行研究、改进，以期建立一种操作简便，结果可信、肺癌检出率高的检查方法。本文采用组合单克隆抗体，经醋酸纤维薄膜电泳法，对７０例肺癌患者血清及１００例正常人血清进行了检测。结果显示，肺癌血清中癌相关抗原的检出率为８３％，正常人血清组的假阳性率为１０％，误诊率小于３０％，故此方法有诊断应用价值。Lung cancer is one of the most common cancers in our country. We constantlystudy to improve the diagnostic methods of lung cancer in order to establish a simple, reliable andhigh detection method. In this paper 70 cases of serum in patient with lung cancer and 100 casesof serum in normal individual were detected by electrophoresis and enzyme staining using monocl-onal antibodies by “cock-tail”. The results showed that the positive rate of tumor associated antigen(TAA) in the lung cancer cases was 83% while positive rate of TAA in the normal individualwas l0%. The diagnostic mistake rate(false negative rate plus false positive rate) was less than3o%. Therefore it is an useful method to serological diagnosis in lung cancer.

关 键 词：肺肿瘤 血清学诊断 单克隆抗体 醋酸纤维薄膜电泳 

分 类 号：R734.204[医药卫生—肿瘤]