大鼠血管球囊损伤促进血小板活化、凝血酶受体及血管紧张素Ⅱ1型受体的mRNA表达  

Promotion of the expression of thrombin receptor mRNA and angiotensin Ⅱ AT_1 receptor mRNA, as well as platelet activation by the damage of the aortic wall of rat

作　　者：李永红[1] 葛志明[1] 李志强 郭明磊[3] 董果雄[3] 李震海[4] 张运[1] 

机构地区：[1]山东大学齐鲁医院心内科,山东济南250012 [2]青岛市中级法院技术处,山东青岛266071 [3]青岛大学医学院附属医院,山东青岛266003 [4]青岛市卫生局卫生监督所,山东青岛266033

出　　处：《基础医学与临床》2005年第5期468-472,共5页Basic and Clinical Medicine

摘　　要：目的该研究通过建立大鼠主动脉内皮球囊损伤模型,观察血小板活化、凝血酶受体及血管紧张素Ⅱ1型受体的变化。方法将大鼠随机分为对照组(n=24)和手术组(n=24),并于术后3、7、14和28d取主动脉,通过组织学检查、放射免疫法和反转录聚合酶链反应(RT-PCR)技术检测血管球囊损伤后内膜增生的过程、血小板表面GMP-140的数目、血管AT1受体和凝血酶受体mRNA表达的变化。结果①凝血酶受体mRNA在正常血管组织表达极弱,球囊损伤术后3d已显著增加,术后14d达峰值,术后28d开始下降。②AT1受体mRNA于术后3d明显增高,并持续至术后14d,术后28d恢复至对照水平。③GMP-140于术后3d明显升高,术后7d开始下降。④内皮损伤术后3d已有增殖的血管平滑肌细胞(VSMC)移行至内膜层,术后7d内膜开始增生,术后14dVSMC的增殖及内膜增生更为明显,术后28dVSMC的增殖明显减弱,细胞外基质增加,内膜继续增生。结论血小板活化、凝血酶受体和AT1受体mRNA表达增加参与了血管内皮损伤后内膜增生的过程。ObjectiveTo investigate the mechanism of acute and late restenosis after percutaneous transluminal coronary angioplasty(PTCA)by examination of the process of neointimal formation, level of platelet activation, expression of angiotensin AT_1 receptor mRNA and thrombin receptor mRNA. MethodsForty eight male wistar rats were randomly divided into group 1 (n=24) which served as controls and group 2 (n=24) in which aortic balloon injury by 2F balloon catheter was performed. The process of intimal thickening, the number of platelet GMP-140, the level of AT_1 receptor mRNA and thrombin receptor mRNA were evaluated at day 3, 7, 14 and 28 after balloon injury. ResultsA low expression of thrombin receptor mRNA in endothelium and smooth muscle cells of normal arteries, which increased significantly at day 3 after balloon injury, reached its peak at day 14 and decreased at day 28. The expression of AT_1 receptor mRNA also increased significantly at day 3 after balloon injury, remained high at day 14 and restored to the level of controls atday 28. The number of platelet GMP-140 was increased at day 3 and began to decrease at day 7 after injury. The migration and proliferation of vascular smooth muscle cells(VSMC) had existed at day 3 after balloon injury. The intimal thickening began at day 7 after injury, and it was more significant at day 14. The proliferation of VSMC decreased at day 28, but extracellular matrix increased and the intimal thickening continued. ConclusionThe increased expression of thrombin receptor mRNA, AT_1R mRNA and the number of platelet GMP-140 participate in the process of intimal thickening after vascular balloon injury.

关 键 词：MRNA表达 凝血酶受体 血管球囊损伤 血小板活化 大鼠 血小板表面GMP-140 血管紧张素Ⅱ1型受体 反转录聚合酶链反应 AT1受体mRNA 血管平滑肌细胞 内膜增生 血管内皮损伤 VSMC 主动脉内皮 组织学检查 放射免疫法 细胞外基质 

分 类 号：R543[医药卫生—心血管疾病]