部分背根切断对备用背根节神经元EGF表达的影响  

Expression of EGF in Spared Dorsal Root Ganglion after Partial Dorsal Root Rhizotomy

作　　者：张连双[1] 王希锋[1] 殷彦君[1] 徐新运[2] 章为[2] 王廷华[3] 

机构地区：[1]滨州医学院组胚教研室,山东滨州256603 [2]四川大学组胚与神经生物学教研室,四川成都610041 [3]昆明医学院神经科学研究所,云南昆明650031

出　　处：《海南医学》2005年第6期124-125,共2页Hainan Medical Journal

摘　　要：目的研究部分去背根对备用背根节神经元表皮生长因子(EGF)表达的影响。方法制作猫备用背根模型,在3d、7d及14d时取备用背根节用EGF抗血清(1∶200)行免疫组织化学ABC法染色。分别计数各组大、中小及总阳性神经元的数量。结果EGF在备用背根节的大、中小神经元均有表达。EGF总、大、中小阳性神经元数3d时较正常均明显减少(P<0.05),7d时,总阳性神经元与正常相比无差异,大阳性神经元较正常明显增加(P<0.05),而中小阳性神经元较正常继续减少(P<0.05),14d时均又与正常无差异。结论部分背根切断对备用背根节神经元EGF总、大及中小阳性神经元的表达有不同的影响。但都经历了3d时较正常减少,14d恢复至正常水平的变化趋势。Objective To investigate the expression of epidermal growth factorEGF in spared dorsal root ganglionDRG after partial dorsal root rhizotomy. Methods Twenty adult cats were divided into four groups. Three groups were subjected to bilateral spared root rhizotomy L1～L5 L7～S2 DRGs were sectioned L6 DRG was spared. On the 3rd day 7th day and 14th day after partial dorsal rhizotomy the animals of these groups and another group  intact animals as reference group were sacrificed. The spared DRGs L6 were taken and made into frozen sections and were stained under the same condition using specific EGF antibody1200 by immunohistochemical ABC method. The EGF positive total large medium-small sized neurons in spared DRG were counted respectively. Results All of the EGF positive total large and medium-small sized neurons decreased greatly at 3th days P<0.05 and close to the normal level at 14th daysP>0.05. At 7th days compared with normal group positive total neurons have no difference large neurons increase and medium-small sized neurons continue to decrease. Conclusions partial dorsal root rhizotomy have different influence on the expression of EGF in spared DRG of the total large and medium-small sized neurons.

关 键 词：EGF 备用背根节 免疫组织化学 

分 类 号：R-33[医药卫生]