特异性Tetrodotoxin亲和吸附微珠的制备及其提取性能  

Studies on the Preparation and Characteristics of the Tetrodotoxin-Specific Adsorption Microspheres

作　　者：陈树桥[1] 赵清良[1] 唐梓进[2] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]江苏吴中大自然生物工程公司,江苏南京210097

出　　处：《南京师大学报（自然科学版）》2005年第2期92-95,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏吴中大自然生物工程有限公司资助项目

摘　　要：将横纹东方豚卵巢与醋酸乙醇溶液混合匀浆,经离心,加热,沉淀,脱脂等处理,制得粗提液,活性炭吸附粗提液得到粗毒液;以壳聚糖为载体合成亲和微珠分别来分离纯化粗提液和粗毒液,用传统的小鼠生物法检测TTX毒素量.潮湿活性炭对TTX的吸附量约为2μg/g,潮湿亲和微珠吸附量约为10μg/g;亲和微珠吸附提取粗毒液,回收率达51%,直接吸附粗提液,回收率达到80%,同时蛋白也得到有效的去除.The crude extraction of TTX by aceticacid-ethanol is obtained from the ovary of Fugu oblongus, then de-fatted with chloroform. The crude extraction of TTX is absorbed by activated charcoal and futher purified by new kind of the affinity adsorption chitosan microspheres.The adsorption of tetrodotoxin is measured with the mouse bioassay. The adsorptive c-apacity of affinity adsorption chitosan microspheres reaches about 10μg TTX/g(wet),the reco-very rate is about 51% and 80%,and the protein concentration is lowered about 3 and 4 times.The efficiency of purrification and the recovery by affinity adsorption chitosan microspheres are higher than by the activated charcoal. The chitosan microspheres can be reused after regeneration.

关 键 词：河豚毒素 壳聚糖微珠 亲和吸附 小鼠生物检测 

分 类 号：R318[医药卫生—生物医学工程]