地塞米松对哮喘豚鼠肺及内脏感觉传入部位IL-1β表达的抑制作用  

The inhibitory effect of dexamethasone on the expression of IL-1βin the lung and the visceral sensory afferent system of asthmatic guinea pigs

作　　者：曹德寿[1] 佟浩[1] 刘晓湘[2] 刘丽[2] 方秀斌[2] 

机构地区：[1]中国医科大学基础医学院解剖学教研室 [2]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001

出　　处：《中国药理学通报》2005年第6期681-684,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金(No30070330);辽宁省教育厅基金(No20121032)资助项目

摘　　要：目的探讨地塞米松对哮喘豚鼠肺及内脏感觉传入系统(C7T5脊神经节及对应的脊髓后角)IL-1β表达的抑制作用。方法以卵蛋白致敏豚鼠制作哮喘模型,免疫组织化学检测各组豚鼠C7T5脊神经节及对应的脊髓后角IL-1β的表达,Westernblot免疫印迹检测各组豚鼠肺、C7T5脊神经节及对应的脊髓后角IL-1β的蛋白表达变化。结果哮喘组豚鼠IL-1β在肺及内脏感觉传入系统表达明显高于对照组(P<0.01),而地塞米松组则明显低于哮喘组(P<0.01)。结论IL-1β可能参与哮喘的发病过程,地塞米松抑制哮喘豚鼠IL-1β的表达可能是激素治疗哮喘的机制之一。Aim To explore the inhibitory effect of dexamethasone on the expression of IL-1β in the lung and the visceral sensory afferent system(C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord) of asthmatic guinea pigs. Methods Experimental asthma model of guinea pigs was induced by ovalbumin in vivo. The expression of IL-1β was detected by means of immunohistochemistry in the C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord of all guinea pigs. The expression of IL-1β protein was investigated by Western blot in the lung, C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord of all guinea pigs. Results The expression of IL-1β in the lung, C_7-T_5 spinal ganglia and the corresponding posterior horn of the spinal cord of the asthmatic guinea pigs was much higher than that of the control groups(P<0.01) and the expression of IL-1β of the dexamethasone group was much lower than that of the asthmatic group (P<0.01). Conclusions The present results indicate that the over-expression of IL-1β might be involved in the pathogenesis of the bronchial asthma, and inhibition of the expression of the IL-1β by dexamethasone maybe one of the mechanisms in the treatment of asthma.

关 键 词：白介素-1Β 地塞米松 肺 脊神经节 脊髓 哮喘 

分 类 号：R-332[医药卫生] R322.35