粉防己碱对喹啉酸致海马神经元损伤的保护作用  

Protective effect of Tet on cytotoxicity-induced by Qinolinic acid in cultured hippocampal neurons of new-brone rats

作　　者：朱丽霞[1] 董志[1] 周歧新[1] 廖红[1] 

机构地区：[1]重庆医科大学药理学教研室,生物化学和分子药理学重点实验室,重庆400016

出　　处：《中国药理学通报》2005年第6期718-720,共3页Chinese Pharmacological Bulletin

基　　金：重庆市科研基金资助项目(No渝科发计字[2000]18号)

摘　　要：目的研究粉防己碱(Tet)对喹啉酸(QA)诱导原代培养乳鼠海马神经元兴奋毒损伤的保护作用。方法乳鼠海马神经元原代培养后用QA诱导损伤,MTT法测定细胞活力,同时测定培养上清中LDH活性,神经元病理染色后进行形态学观察。结果Tet(10-6mol·L-1和10-7mol·L-1)能明显提高QA损伤后原代培养乳鼠海马神经元的细胞活力,降低其上清液中LDH活性,与模型组相比,差异均有显著性(P<0.01),并明显改善神经元病理损伤。结论Tet(10-6mol·L-1和10-7mol·L-1)对QA所致的原代培养乳鼠海马神经元损伤有明显保护作用。Aim To study the protection of tetrandrine(Tet) from the excitotoxicity of quinolinic acid(QA) on the cultured hippocampal neurons of new-borne rats.Method Excitoxocity injury was induced by QA in cultured hippocampal neurons.The cell viability was measured by MTT assay, extracellular LDH contents were also measured and the pathomorphology of neurons was observed. Result Tet with 10 -6 mol·L -1 and 10 -7 mol·L -1 could significantly blunt the death of the primary hippocampal neurons induced by QA,prevent leakage of LDH and improve the pathomorphology of neurons. Conclusion Tet (10 -6 mol·L -1 and 10 -7 mol·L -1)provids protection on QA induced cytotoxicity in cultured hippocampal neurons of new-borne rats.

关 键 词：粉防己碱 海马神经元 喹啉酸 兴奋毒损伤 

分 类 号：R-332[医药卫生] R284.1