恶性肿瘤患者胸腹水中巨噬细胞的功能观察——细胞毒性作用、白细胞介素1产生的能力  

FUNCTIONAL PROPERTIES OF HUMAN EFFUSION MACROPHAGES FROM PATIENTS WITH MALIGNANT TUMOR—CYTOTOXICITY AND IL-1 PRODUCTION

作　　者：陈秋艳[1] 袁定东[1] 

机构地区：[1]西安医科大学病理解剖学教研室

出　　处：《西安医科大学学报》1989年第4期320-323,共4页Journal of Xi'an Medical University(Chinese)

基　　金：国家自然科学基金

摘　　要：为探讨人肿瘤相关巨噬细胞(TAM)在肿瘤发展中的作用,在体外研究了20例癌性胸、腹水中巨噬细胞(Mφ)的功能,包括 IL-1的产生和对人肺癌细胞系PGT 的细胞毒作用,用12例结核性胸水中之 Mφ作为对照。实验表明:TAM 的IL-1产生能力及细胞毒作用均较结核性胸水 Mφ为低;LPS、IFN 或消炎痛可增强 TAM 的此等功能;患者自身胸、腹水于高浓度时(≥12.5%)抑制而于低浓度时(6.35%)增强此等 TAM 功能。实验提示 TAM 中抑制性 Mφ增多、一些 TAM 处于未活化状态和癌性积液中存在的某些其它抑制因子均可能与 TAM 功能降低有关.In order to explore the role of human tumor-associated macrophages(TAM)on tumor development in vitro functional activities,including interleukin-1(IL-1)production and cytotoxicityagainst a human lung tumor cell line PGT,ofmacrophages(Mφ)isolated from 20 cases of cancerouspleural or peritoneal effusions were studied,Mφ from12 cases of tuberculous pleural effusions were used ascontrols.Our results showed that both IL-1 produc-tion and cytotoxicity of TAM were decreased as com-pared with Mφ of tuberculous effusions;LPS,IFN orindomethacin could enhance the TAM functions;autologus effusion of cancer patients at higher concentra-tion(≥12.5%)inhibited the TAM functions,but en-hanced them at lower concentration(6.25%).Theseresults suggest that more suppressor Mφ amongTAM,some TAM in an unactivated state,and someother inhibitors in cancerous effusion all may be respon-sible for the reduced TAM function.

关 键 词：癌 腹水 巨噬细胞 白细胞介素 胸水 

分 类 号：R730.3[医药卫生—肿瘤]