不同剂量脑醒喷鼻剂对再灌注损伤脑组织自由基及一氧化氮合酶变化的干预(英文)  

Changes of free radicals and nitric oxide synthase in rat brain following ischemia-reperfusion injury due to different dosage brainawakening nasal sprayer intervention

作　　者：李荣[1] 吴伟[1] 陈宏珪[1] 黄衍寿[1] 刘煜德[1] 杨开清[1] 

机构地区：[1]广州中医药大学第一附属医院内科,广东省广州市510405

出　　处：《中国临床康复》2005年第21期254-256,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省中医药管理局资助课题(98342)~~

摘　　要：背景:脑醒喷鼻剂由川芎和石菖蒲等中药组成,《神农本草经》谓其“主脑卒中入脑”之功效。目的:观察脑醒喷鼻剂对大鼠局灶性脑缺血再灌注损伤脑组织自由基及一氧化氮合酶变化的影响,并与经典西药尼莫地平相比较。设计:随机对照实验。单位:一所中医药大学医院的内科。对象:清洁级成年雄性Wistar大鼠70只,随机分为7组:脑醒喷鼻剂高剂量组(高剂组),脑醒喷鼻剂中剂量组(中剂组),脑醒喷鼻剂低剂量组(低剂组),尼莫地平腹腔注射组(尼莫地平组),生理盐水喷鼻剂组(生理盐水组),溶酶喷鼻剂组(溶酶组),假手术组,每组10只。方法:除假手术组外,其他6组阻断大鼠左侧大脑中动脉建立大鼠局灶脑缺血再灌注模型。在造模前3d及再灌注期间,脑醒喷鼻剂高、中、低剂组分别以含川芎嗪和石菖蒲生药5.4,2.7,1.08mg/(kg·d)和1.35,0.54,0.27g/(kg·d)的剂量滴鼻,3次/d;生理盐水组和溶酶组以生理盐水、溶酶喷鼻剂0.18mL/(kg·d)滴鼻,3次/d;尼莫地平组用尼莫地平注射液0.8mg/(kg·d)腹腔注射,2次/d;假手术组按常规饲养至实验取材。用比色法检测丙二醛、超氧化物歧化酶及一氧化氮合酶。主要观察指标:①不同剂量脑醒喷鼻剂及其他组动物脑组织丙二醛、超氧化物歧化酶及一氧化氮合酶活性变化。②将不同剂量脑醒喷鼻剂组与尼莫地平组做比较。结果:造模时死亡8只动物,进入结果分析62只。①丙二醛含量:高剂组、尼莫地平组明显低于生理盐水组[(0.92±0.32),(0.87±0.39),(1.35±0.34)μmol/g,P<0.05],但高剂组和尼莫地平组间无差异。②超氧化物歧化酶活性:高剂组、尼莫地平组明显高于生理盐水组[(35.64±11.67),(33.88±7.15),(20.70±3.88)NU/mg,P<0.05],但高剂组和尼莫地平组间无差异。③一氧化氮合酶活性及超氧化物歧化酶活性:高剂组、尼莫地平组明显高于生理盐水组[(4.64±1.22),(5.00±1.10),(3.08±1.12)mkat/g,BACKGROUND:Brainawakening nasal sprayer is composed of many herbs,such as Chuanxiong and Shichangpu,which were regarded by “Shennong Bencaojing' as having the function of “preventing stroke in the brain'.OBJECTIVE:To observe the changes of free radicals and nitric oxide synthase in rat brain following focal ischemicreperfusional injury due to brainawakening nasal sprayer intervention and compare with that due to classical nimodipine.DESIGN:A randomized controlled study.SETTING:Department of internal medicine of a hospital affiliated to a traditional Chinese medical university.MATERIALS:Seventy adult male Wistar rats of clean grade,were randomly divided into seven groups:brainawakening nasal sprayer of higher dosage group,moderate dosage group,lower dosage group,nimodipine intraperitoneal injection group,physical saline nasal sprayer group,menstruum nasal sprayer group,and sham operation group with 10 rats in each.METHODS:Focal brain ischemiareperfusion model was established by blocking the left cerebral middle artery in rats of all the groups except sham operation group.Three days before model establishment and during reperfusion,rats were given different dosages of brainawakening nasal sprayer composed of Chuanxiongqin and Shichangpu of 5.4,2.7,1.08 mg/(kg·d) and 1.35,0.54,0.27 g/(kg·d),respectively,three times a day;which was replaced by physical saline and menstruum nasal sprayer of 0.18 mL/(kg·d),three times a day in physical saline nasal sprayer group and menstruum nasal sprayer group;rats in nimodipine intraperitoneal injection group received intraperitoneal injection of nimodipine by 0.8 mg/(kg·d) twice a day.Rats in sham operation group were routinely raised.The content of prodialdehyde,superoxide dismutase and nitric oxide synthase were measured with colorimetric method.MAIN OUTCOME MEASURES:①The changes of prodialdehyde content,superoxide dismutase and nitric oxide synthase activity in rat brain following focal ischemicreperfusional injury in groups of different dosage of

关 键 词：脑缺血/中药疗法再 灌注损伤/中药疗法 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶/药物作用 一氧化氮合酶/药物作用 投药 鼻内 

分 类 号：R743[医药卫生—神经病学与精神病学]