脊髓腹侧急性损伤受压模型的病理变化  

Pathological changes of acute ventral compression injury model of spinal cord

作　　者：蒙树岳[1] 庄小强[2] 陆生林[2] 胡日焕[2] 莫洪耀[2] 莫忠贵[2] 胡进[2] 程军[2] 莫维光[3] 文宗振[4] 

机构地区：[1]广西壮族自治区江滨医院骨科,广西壮族自治区南宁市530021 [2]广西民族医院骨科,广西壮族自治区南宁市530001 [3]广西医科大学第一附属医院病理科,广西壮族自治区南宁市530021 [4]广西大学电子显微镜室,广西壮族自治区南宁市530001

出　　处：《中国临床康复》2005年第21期28-29,i001,共3页Chinese Journal of Clinical Rehabilitation

基　　金：崇左市科技局科研经费资助的2001~2002年科技攻关项目(012001)~~

摘　　要：目的:构建一种和临床患者相似的脊髓腹侧受压损伤模型,并观察在排除实验因素的影响下脊髓损伤早期的病理变化过程。方法:实验于2001-08在广西民族医院动物实验室完成。将25只广西成年健康山羊随机分成5组:压迫2h组、压迫4h组、压迫6h组、压迫8h组、压迫10h组,每组5只。于T13椎体中后部额状面切骨,咬除椎弓根,在切骨线打入木条使椎体后缘突入椎管,造成前1/3占位,持续全麻固定,于压迫2,4,6,8和10h切取脊髓,造成脊柱骨折后脊髓腹侧急性损伤受压模型。大体观察和在光学显微镜和电子显微镜下观察脊髓出血、变性及坏死的情况。结果:每组有5只山羊进入结果分析。①大体观察:压迫2h组脊髓有轻度的出血、坏死;压迫8h组出血、水肿、坏死加重,神经元、胶质细胞肿胀;压迫10h组肉眼观察已有胶冻样改变。②光镜下可见:神经元部分崩解。结论:脊髓腹侧损伤被持续压迫2h时已有轻度病理变化,随着压迫时间的延长,出血、水肿、坏死液化呈现进行性加重,至10h时,脊髓坏死已很严重。实验中脊髓腹侧受压模型和脊髓病理变化和临床中的情况一致,重复性、定量性好,是一个较理想的脊髓损伤模型。AIM:To establish a ventral compression injury model of spinal cord,which was similar to the symptoms of the clinical patients,and observe the pathological changes of early spinal cord injury except for experimental factors. MEHTODS:The experiment was finished in the Laboratory of Animal of Guangxi National Hospital in August 2001.Twentyfive Guangxi adult goats were divided randomly into five groups(n=5):2,4,6,8 and 10hour compression groups.T13 vertebral body of all goats was cut in frontal posterior part and the pedicle of vertebra was excised.A wood strip was inserted into the incision to force the posterior border of vertebral body into the 1/3 of vertebral canal under durative anaesthesia. The spinal cord was cut at 2,4,6,8 and 10 hours to establish ventral compression injury model of spinal cord.The hemorrhage,degeneration and necrosis of spinal cord were observed by using naked eye,light microscope and electron microscope. RESULTS:All animals were involved in the result analysis.①Slight hemorrhage and necrosis of spinal cord were observed in 2hour compression group.The hemorrhage,edema and necrosis became serious, and neurons and glial cell swelled in 8hour compression group.Colloidal change was observed with naked eye in 10hour compression group.②Some cracked neurons were found under light microscope. CONCLUSION:Slight pathological changes are found 2 hours after persistently ventral compression of spinal cord,and along with the prolonged time.The hemorrhage,edema and necrosis become more serious and reach to the worse condition at 10hour compression group.The ventral compression injury model of spinal cord and pathological changes in experiment are coincident with those in clinic.The model with good repetitive and quantitative characters is an ideal model.

关 键 词：脊髓损伤 疾病模型 动物 病理学 

分 类 号：R744[医药卫生—神经病学与精神病学]