两种髓鞘蛋白脂质蛋白肽段诱导自身反应性脑脊髓炎实验动物模型的比较(英文)  

Comparison of experimental autoimmune encephalomyelitis models induced by two kinds of proteolipid proteins

作　　者：张金涛[1] 朱克[2] 金香兰[1] 邢广羽[1] 张笑明[1] 倪建强[1] 宋春杰[1] 尹岭[1] 

机构地区：[1]解放军总医院神经信息中心,北京市100853 [2]解放军总医院神经病学研究所,北京市100853

出　　处：《中国临床康复》2005年第21期225-227,i004,共4页Chinese Journal of Clinical Rehabilitation

基　　金：全军十五医药卫生科研基金资助项目(01Z041)~~

摘　　要：背景:实验性自身免疫性脑脊髓炎小鼠模型是多发性硬化症的经典动物模型,目前国内的模型多呈现单向病程。应用髓鞘蛋白脂质蛋白139～151和髓鞘蛋白脂质蛋白178～191诱导具有免疫易感性的SJL/J小鼠,有可能建立与多发性硬化不仅临床表现及病理反应接近,而且病程相似的复发缓解型自身免疫性脑脊髓炎的动物实验模型。目的:建立与多发性硬化不仅临床表现、病理接近,而且病程相似的较为理想的复发缓解型实验性自身免疫性脑脊髓炎模型。设计:完全随机设计的对照实验。单位:解放军总医院神经信息中心和神经病学研究所。材料:实验于2004-02/06于解放军总医院神经病理研究室进行。选用8～12周龄雌性SJL/J小鼠60只,随机分为髓鞘蛋白脂质蛋白139～151和髓鞘蛋白脂质蛋白178～191组各30只。干预:应用髓鞘蛋白脂质蛋白139～151和髓鞘蛋白脂质蛋白178～191免疫雌性SJL/J小鼠,制作复发缓解型实验性自身免疫性脑脊髓炎模型,观察其体质量及神经功能评分的变化,应用苏木精-伊红,Luxol固蓝髓鞘染色等方法观察模型的组织形态学改变。主要观察指标:①两种髓鞘蛋白脂质蛋白肽段免疫的小鼠出现缓解-复发的特点以及明显的神经系统体征。②病理学及体质量和神经功能变化评分。结果:全部60只小鼠均纳入分析。①两种髓鞘蛋白脂质蛋白肽段免疫的小鼠发病均具有缓解-复发的特点,出现明显的神经系统体征;小鼠发病时脑和脊髓组织显示明显的血管鞘形成、卫星现象和炎性细胞浸润,以及脱髓鞘改变。②体质量变化:免疫前两组小鼠体质量为(17.84±2.59),(17.88±0.52)g。髓鞘蛋白脂质蛋白178～191组发病早,恢复快,免疫后小鼠体质量增加减缓,60d内体质量增长到(23.52±2.37)g。髓鞘蛋白脂质蛋白139～151组发病晚,恢复相对较慢,免疫后小鼠体质量较免疫前略有下降,60d时体质�BACKGROUND:Experimental autoimmune encephalomyelitis has become the most classical animal model for multiple sclerosis.However,the experimental autoimmune encephalomyelitis model of China presented oneway course of disease.By using proteolipid protein 139-151 and proteolipid protein 178-191,relapse remitting experimental autoimmune encephalomyelitis models may be induced in SJL/J mice which were susceptible to immune, which have similar clinical situation,course and histological alterations to multiple sclerosis.OBJECTIVE:To establish the relapse remitting experimental autoimmune encephalomyelitis mouse model induced by proteolipid protein,which has similar clinical situation,course and histological alterations to multiple sclerosis.DESIGN:Completely randomized controlled study.SETTING:The centre of Neuroinformation,and Neurological Institute, General Hospital of Chinese PLA.MATERIALS:The study was carried out at the Laboratory of Neuropathology, General Hospital of Chinese PLA,from February to June 2004. Sixty female SJL/J mice with 8-12 weeks old were selected and randomly divided into proteolipid protein 139-151 group and proteolipid protein178191 group with 30 in each.INTERVENTIONS:After injected with proteolipid protein139151 or proteolipid protein178191,the models of relapse remitting experimental autoimmune encephalomyelitis were induced, and the body weight and neurological signs of each female SJL/J mouse were viewed.The tissue morphological changes of models were observed with hematoxylin and eosin and Luxol fast blue stain.MAIN OUTCOME MEASURES:The neurological symptoms and signs, features of relapse and remitting and the perivascular inflammatory cell infiltration,demyelinated lesion of the model of experimental autoimmune encephalomyelitis mouse induced by two proteolipid protein peptides.RESULTS:All 60 mice entered the final analysis.①Significant neurological symptoms,signs and features of relapse and remitting was manifested in the model of experimental autoimmune encep

关 键 词：脑脊髓炎 实验性自身免疫性 疾病模型 动物 髓磷脂碱性蛋白质类 多发性硬化 

分 类 号：R743[医药卫生—神经病学与精神病学]