血管内皮生长因子反义核糖核酸对人肺癌的抗血管生成作用  

The anti-angiogenesis effect in lung cancer by antisense RNA of vascular endothelial growth factor

作　　者：刘毅梅[1] 张鹏[1] 宋世辉[1] 张文志[1] 张维铭[1] 

机构地区：[1]天津医科大学总医院心胸外科,300052

出　　处：《中华实验外科杂志》2005年第7期790-791,共2页Chinese Journal of Experimental Surgery

基　　金：天津市自然科学基金资助项目(013611811)

摘　　要：目的探讨血管内皮生长因子(VEGF)反义RNA封闭内源性VEGF表达对人肺癌裸鼠皮下移植瘤血管生成的影响。方法采用30只裸鼠建立人肺癌皮下移植瘤模型,通过原位分子杂交法测定VEGFmRNA、免疫组织化学法测定VEGF表达和微血管密度计数,比较治疗组和空载组、对照组间的差异,研究以脂质体介导VEGF反义cDNA经局部多点注射封闭内源性VEGF表达的抗血管生成作用。结果治疗组肿瘤组织的VEGFmRNA阳性细胞数为(21.83±13.47)个/0.05mm2、VEGF蛋白阳性细胞数(20.76±15.69)个/0.05mm2、微血管密度计数(MVD)为(2.30±1.95)条/0.20mm2,与对照组、空载组的差异均有统计学意义(P<0.01),VEGF反义RNA治疗能够有效的封闭内源性VEGF的生成、使肿瘤组织微血管生成减少,实验结束时瘤体大小与对照组和空载组的差异均有统计学意义(P<0.001)。结论VEGF反义RNA治疗是一种有效的肿瘤抗血管生成基因治疗方法。Objective To study the anti-angiogenesis effect of the blockage of endogenous VEGF production by vascular endothelial growth factor (VEGF) antisense RNA in xenograft lung tumors in nude mice.[WT5”HZ]Methods The models of xenograft lung tumors in 30 nude mice were treated with intra-tumor injection of VEGF antisense cDNA and Lipofectamine complex.VEGF mRNA was investigated by hybridyzation in situ and VEGF protein and MVD by immunohistochemistry in the tumor.The tumor mass was observed.Results Compared to the control,less VEGF mRNA (21.83± 13.47) positive cells/ 0.05 mm2 and protein production (20.76± 15.69) positive cells/ 0.05 mm2,less MVD (2.30± 1.95) microvessells/ 0.20 mm2 were found in the tumor treated by VEGF antisense RNA,and tumor growth was also inhibited.All the differences had statistical significance (P< 0.01).Conclusion Using VEGF antisense RNA to block endogenous VEGF production is an operative gene therapy in treating tumor through anti-angiogenesis.

关 键 词：抗血管生成作用 人肺癌 反义核糖核酸 血管内皮生长因子(VEGF) 微血管密度计数 VEGF表达 反义RNA 裸鼠皮下移植瘤 原位分子杂交法 免疫组织化学法 反义CDNA VEGF蛋白 基因治疗方法 mRNA 肿瘤组织 移植瘤模型 脂质体介导 

分 类 号：R734.2[医药卫生—肿瘤]