高效液相色谱电化学检测生物样品中儿茶酚胺的研究  

Determination of catecholamines in biological samples by Using High Performance Liquid Chromatography with electrochemical Detection

作　　者：李炳源[1] 黄慧伟[2] 黄彦[2] 李新玲[1] 邱一华[2] 

机构地区：[1]南通大学公共卫生学院 [2]南通大学基础医学院生理学教研室,南通226001

出　　处：《南通大学学报（医学版）》2005年第3期177-179,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省高校自然科学基金(04KJA180110);南通市科技项目(S40057)资助项目。

摘　　要：采用AtlantisC18反相色谱柱,成功地分离去甲肾上腺素、肾上腺素、多巴胺等儿茶酚胺类物质。研究了儿茶酚胺在AtlantisC18和NovlepakC18柱的保留特性,比较了流动相中离子对试剂辛烷基磺酸钠(B8)浓度对儿茶酚胺容量因子k'的作用,确认采用AtlantisC18柱分离儿茶酚胺,色谱条件优化空间更大,更适于复杂生物样品分析。色谱条件:AtlantisC18柱,电化检测,流动相:NaH2PO450mmol/L、柠檬酸钠50mmol/L缓冲液-乙腈(体积比为75∶25),检测限(S/N)在0.93～2.43pg以下,绝对回收率均≥98.5%,样品日内相对标准差<1.2%。方法精密、准确。A method for the determination of noradreline(NA),adrenaline(A) dopamine (DA) by using Atlantis C18 column was investigated.The characteristics of retention of catecholamines (CAs) in Atilntis and Novle Pak C18 column were observed,and the contribution of deffrent concentrations of octanesufonat sodium (B8) in mobile phase to capacity factor k' was compared. We proved that it was better to use Atilantis C18 for separating CAs in optimizing chromatographic system, and more suitable to separating the complicated biologic samples particularly.Chromatography condition: the mobile phase was a mixture of buffer and acetonitrile (V∶V=75∶25) and the buffer was NaH2PO4 50mmol/L and citric acid 50mmol/L.The detection limimits of the method were 0.93 to 2.43 pg. The recoveries were above 98.5% and the relative standard deviation (RSDs)of intra-day were all within 1.2%. The method has advantages of high sensitivity and accuracy.

关 键 词：高效液相色谱电化学检测 儿茶酚胺类物质 mol/L 去甲肾上腺素 生物样品分析 C18柱 离子对试剂 容量因子 条件优化 色谱条件 柠檬酸钠 流动相 色谱柱 多巴胺 磺酸钠 柱分离 缓冲液 检测限 回收率 标准差 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R135[医药卫生—临床医学]