地高辛配基人乳头瘤病毒DNA探针的制备和应用  

Preparation of Digoxigenin-HPV DNA Probe and Its Application in Carcinoma of the Cervix

作　　者：钱荆[1] 张幼辰[1] 俞顺章[1] 陈贻训 周元 

机构地区：[1]上海医科大学预防医学研究所,江西省妇产科医院

出　　处：《标记免疫分析与临床》1994年第1期9-12,共4页Labeled Immunoassays and Clinical Medicine

基　　金：卫生部科研基金

摘　　要：采用非同位素标记物地高辛配基（Ｄｉｇｏｘｉｇｅｎｉｎ），通过随机引物法制成地高辛配基人乳头瘤病毒ＤＮＡ（Ｄｉｇ－ＨＰＶＤＮＡ）探针。用斑点杂交法检测宫颈组织中的ＨＰＶＤＮＡ。经试验证明，该探针检测ＨＰＶＤＮＡ的灵敏度与（３２）Ｐ－ＨＰＶＤＮＡ探针相近。经与（３２）Ｐ－ＨＰＶＤＮＡ探针同时对６５份临床标本进行比较，结果附合率达９５％以上。３５份宫颈癌组织中ＨＰＶ１６／１８型检出率为３４．２９％。Ｄｉｇ标记的探针检测ＨＰＶＤＮＡ特异性高，重复性好，背景显色低。Digoxigenin-human papillomavirus DNA probe was prepared by labeling of digoxigenin with random hexanucleotide primer and was applied to detect HPV DNA in biopsies from the cervix of 65 patients (30 uterine leiomyoma and 35 cervical carcinoma). The positivity for HPV16/18 DNA and HPV6b/11 DNA in cervical carcinoma was 34. 29 % and 3. 33% ,respectively. The sensitivity of dot hybridization with Dig-HPV DNA probe was similar to that of ￣(32)P-HPV DNA probe with a 95% consistency in 65 samples. The results indicate that the detection of HPV DNA with this probe is sensitive, specific and reproducible with a low background.

关 键 词：人乳头瘤病毒 DNA探针 地高辛配基 子宫颈肿瘤 

分 类 号：R737.330.4[医药卫生—肿瘤] R392－33[医药卫生—临床医学]