人前列腺特异抗原（hPSA）的纯化及放射免疫分析的建立  

Purification of Human Prostate Specific Antigen (hPSA) and Development of Radioimmunoassay for hPSA

作　　者：仲伟强[1] 陈莉[1] 王仁芝[1] 

机构地区：[1]中国人民解放军军事医学科学院放射医学研究所

出　　处：《标记免疫分析与临床》1994年第3期160-163,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：本文介绍前列腺特异抗原的纯化，免疫动物得到特异性抗ＰＳＡ抗体，并建成放射免疫分析。方法简便快速，灵敏度较高，准确性及重复性良好。与国外相应试剂盒比较有良好的相关性。Human prostate specific antigen (hPSA) RIA was developed with hPSA and anti-PSA prepared in our laboratory. Its standard curve was linear with a sensitivity of 0.5μg/L. Serum PSA levels of 130 normal males ranged from 0 to 3.5μg/L (1.15±0.93μg/L),which are equiralent to the results of other conventional RIA. The recovery,intra-and inter-assay coefficients of variation conformed to the demands of RIA,and the results of 41 samples obtained by both the PSA RIA and PSA of DPC were well correlated (r=0.990).PSA level of 23 patients with prostatic carcinoma was 10～400μg/L.

关 键 词：前列腺特异抗原 放射免疫分析 前列腺肿瘤 

分 类 号：R737.250.4[医药卫生—肿瘤] R730.43[医药卫生—临床医学]