减毒沙门菌体内介导基因转移对ACC-M抑制的实验研究  

A Study of Attenuated Salmonella-mediated Gene Inhibitor Effect on the Lungs Metastasis Transplant Tumor in BALB/C Nude Mice with Human ACC-M

作　　者：江中明[1] 赵平[2] 曹志中[1] 刘军[1] 刘宏[1] 周中华[1] 吕春堂[1] 

机构地区：[1]第二军医大学长海医院口腔科,上海200433 [2]第二军医大学基础医学部微生物教研室,上海200433

出　　处：《口腔颌面外科杂志》2005年第2期145-148,共4页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然基金资助项目(30171055)

摘　　要：目的:观察构建含有Tip30与人IFN-γ基因的真核表达及共表达质粒的重组减毒伤寒沙门菌对腺样囊性癌肺转移抑制作用。方法:4周龄BALB/Cnunu雄鼠25只,随机分成5组,每组5只,其中对照组5只(PBS)、单纯减毒沙门菌组5只(SL7207)、携带人IFN-γ基因减毒沙门菌治疗组5只(SL7207/pCI-IFN)、携带Tip30基因减毒沙门菌治疗组5只(SL7207/pCI-Tip30)、携带Tip30与人IFN-γ基因的真核共表达质粒的重组减毒沙门菌治疗组5只(SL7207/pCI-Tip30/IFN),均于尾静脉接种高转移腺样囊性癌(ACC-M)细胞。10d后对照组裸鼠口服PBS,其余各治疗组口服相应的减毒沙门菌,隔周1次,共3次。检测:裸鼠肺转移肿瘤细胞内IFN-γ、Tip30的表达、肿瘤生长体积、瘤体重量、带瘤存活期。结果:在治疗组裸鼠肺转移肿瘤细胞胞浆内有对应表达IFN-γ、Tip30;各治疗组均较对照组肿瘤生长慢,肿瘤体积小,重量轻,抑瘤率高,带瘤存活期长;携带基因治疗组较SL7207治疗组肿瘤生长慢,肿瘤体积小,重量轻,抑瘤率高,带瘤存活期长;联合基因治疗组较单基因治疗组肿瘤生长慢,肿瘤体积小,重量轻,抑瘤率高,带瘤存活期长。结论:减毒伤寒沙门菌不仅能作为载体介导基因对腺样囊性癌肺转移有抑制作用,而且自身对腺样囊性癌肺转移也有抑制作用。联合基因较单基因抑制效果好,有协同作用。Objective: To observe inhibitor of 4 kinds constructed attenuated Salmonella typhimurium on the lungs metastasis transplant tumor growth in BALB/C nude mice with human salivary adenoid cystic carcinoma. Methods: 25 four-week-old BALB/C male nude mice were divided randomly into 5 groups, each group had 5 mice. They were the control group (PBS), the simple attenuated Salmonella group (SL7207), the recombinant attenuated Salmonella typhimuriums haboring plasmid pCI-IFN therapy group(SL7207/pCI-IFN), the recombinant attenuated Salmonella typhimuriums haboring plasmid pCI-Tip30 therapy group (SL7207/pCI-Tip30) and the recombinant attenuated Salmonella typhimuriums haboring co-expressing plasmid pCI-Tip30/IFN therapy group(SL7207/pCI-Tip30/IFN). All groups were injected ACC-M cells suspension at the tail vein. The control group accepted PBS by oral administration 10 days after injection, and the other groups accepted the different recombinant Salmonella typhimuriums respectively, once every 2 weeks and 3 times in all. Then the ACC-M lung metastasis tumor were detected with the expressions of Tip30 and human IFN-γ gene, tumor size, tumor weight, and mice survival period with tumor. Results: There were Tip30 and human IFN-γ gene expression in cytoplasm of ACC-M lungs metastasis transplant tumor cells in all therapy groups. The tumor size and weight of the therapy groups were lower than that of the control group, the rate of suppressing of the therapy groups were higher than that of the control group and mice survival period of therapy groups were longer than the control group. In the therapy groups haboring Tip30 and human IFN-γ gene, the tumor size, tumor weight were lower than that of the SL7207 group, the rate of suppressing were higher than that of SL7207 group and mice survival period were longer than that of the SL7207 group. In the united genes therapy group, the tumor size, tumor weight were lower than that of the single gene groups, the ratio of suppressing tumor were higher than that of the single gene group

关 键 词：介导基因转移 ACC-M 实验研究 IFN-Γ基因 减毒伤寒沙门菌 腺样囊性癌 TIP30基因 重组减毒沙门菌 共表达质粒 肿瘤生长 体内 转移抑制作用 BALB/C 肿瘤体积 治疗组 联合基因 肺转移肿瘤 存活期 对照组 抑瘤率 肿瘤细胞 

分 类 号：R739.8[医药卫生—肿瘤]