褪黑素对大鼠应激性溃疡保护作用研究  

Study of The Preventive Effect of Melation on stress ulcer in Rats

作　　者：李开学[1] 邓联民[1] 陈碧玲[1] 刘新民[1] 

机构地区：[1]广东省深圳市第二人民医院消化科,518035

出　　处：《中国误诊学杂志》2005年第7期1219-1221,共3页Chinese Journal of Misdiagnostics

摘　　要：目的:探讨褪黑素(melatonin,MT)对大鼠应激性溃疡的保护作用及机制。方法:采用浸水-束缚(WIR)应激实验复制大鼠应激性溃疡模型。应激前30 min,MT预防组和预防对照组大鼠分别腹腔注射MT(15 mg/kg)和等体积生理盐水。应激6 h后,观察各组大鼠胃黏膜病变情况,对溃疡指数(U I)进行评分,同时检测各组大鼠胃黏膜血流量,以及黏膜内氨基己糖和前列腺素的含量,并观察组织学改变。结果:WIR应激6 h后,与预防对照组比较,MT预防组大鼠胃黏膜病变明显减轻,U I显著降低(P<0 .0 1) ,胃黏膜血流得到改善,黏膜内氨基已糖和前列腺素的含量均见升高(P<0 .0 1)。结论:MT可有效预防急性胃黏膜损伤的发生,其机制可能与MT增加胃黏膜血流。Objective:To explore the effect of melatonin on stress ulcer in rats and its mechanisms.Methods:Water immersion restrain (WIR) experiment was applied to replicate the stress ulcer model in rats.MT group and control group were given intraperitoneal injection of MT (15 mg/kg) and equal volume physiological saline,respectively,thirty minutes before stress.Six hours after stress,the mucosal changes in each group were obsereved,and the ulcer index score,gastric mucosal blood flow,volume of hexosamine and prostaglandin in the mucosa,and histological changes were evaluated.Results:The mucosal damage six hours after stress was more light in the MT group than that in the control group .Ulcer index decreased significantly(P<0.01),the gastric mucosal blood flow improved and the volume of hexosamine and prostaglandin in the mucus both increased (P<0.01) compared with those in the control group.Conclusions:MT is effective in preventing acute gastric mucosal lesion.The mechanism should be related with the increase of gastric mucosal blood flow and synthesis of hexosamine and prostaglandin by MT.

关 键 词：应激性溃疡 褪黑素 大鼠 作用研究 急性胃黏膜损伤 前列腺素 胃黏膜血流量 氨基己糖 组织学改变 胃黏膜病变 保护作用 溃疡模型 应激实验 生理盐水 腹腔注射 病变情况 溃疡指数 同时检测 氨基已糖 有效预防 对照组 MT 含量 

分 类 号：R573.1[医药卫生—消化系统] R977.1[医药卫生—内科学]