表皮生长因子对肠道放射性损伤保护作用的量效关系(英文)  

Dose-effect relationship between epidermal growth factor and its protection of the intestinal tract from the radioactive damage

作　　者：徐佩茹[1] 李莉[2] 赵效国[2] 刘涛[2] 黄晖[2] 杨剑[2] 

机构地区：[1]新疆医科大学第一附属医院临床营养研究室,新疆维吾尔自治区乌鲁木齐市830000 [2]新疆医科大学公共卫生学院,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国临床康复》2005年第22期223-225,i007,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:表皮生长因子可以抑制胃酸分泌,促进溃疡愈合,对胃肠道黏膜具有保护作用,还具有促进黏膜细胞生长和增殖的作用。目的:观察不同剂量表皮生长因子对放射性肠道损伤后大鼠肠黏膜屏障影响的量效观察。设计:随机对照实验。单位:新疆医科大学第一附属医院及公共卫生学院。材料:实验于2001-08/2003-05在新疆医科大学公共卫生学院和新疆医科大学第一附属医院完成。选用新生Wistar大鼠80只,雌雄各半。方法:将Wistar大鼠随机分成阴性,阳性对照组及表皮生长因子30μg/kg,20μg/kg,10μg/kg组,每组16只,雌雄各半。除阴性对照组外,各组大鼠腹部γ射线单次9.5Gy辐射制备动物模型后,皮下注射表皮生长因子1次/d进行干预,8d后观察动物体质量、食物摄入量、食物利用率的变化,并行肠黏膜形态学检查。主要观察指标:①不同剂量表皮生长因子对放射性损伤后大鼠体质量增长、食物摄入量、每日食物摄入量、每日食物利用率的影响。②光镜和电镜下肠黏膜检查结果。结果:①应用表皮生长因子30,20,10μg/kg组大鼠体质量增长与阴性对照组及阳性对照组比较,差异无明显意义(P>0.05)。②应用表皮生长因子30,20,10μg/kg组雄鼠和雌鼠每日食物摄入量与阴性对照组比较,差异有显著意义(P<0.05),与阳性对照组比较差异无显著意义(P>0.05)。③光镜和电镜结果显示,应用表皮生长因子使放射性肠道损伤后肠黏膜形态结构随着表皮生长因子剂量的增加,逐渐改善,其中表皮生长因子30μg/kg组有明显改善,可见上皮细胞显著增生,杯状细胞数目增多。结论:不同剂量表皮生长因子对大鼠体质量、食物摄入量、食物利用率可能没有影响,但大剂量组表皮生长因子对放射性肠道损伤后肠黏膜形态结构的改善,以及肠上皮细胞增生和修复产生促进作用。BACKGROUND:Epidermal growth factor can inhibit gastric acid secretion,promote the healing from the ulcer,protect mucous membrane,and enhance the growth and proliferation of mucous membrane cells of the gastrointestinal tract. OBJECTIVE:To investigate the effect of epidermal growth factor at different doses on the barrier function of the intestinal mucous membrane in rats after radioactive damage. DESIGN:A randomized controlled trial. SETTING:Department of Clinical Trophology,First Affiliated Hospital and College of Public Health. MATERIALS:The experiment was completed at the Public Health Department of Xinjiang Medical University and the First Affiliated Hospital of Xinjiang Medical University from August 2001 to May 2003.Totally 80 newborn Wistar rats,half male and half female,were involved in the study. METHODS:Wistar rats were randomly divided into the control groups(positive and negative) and intervention groups(30 μg/kg,20 μg/kg,10 μg/kg) with 16 rats in each group and half male and half female.The models were made by a single radiation of 9.5Gy on the abdomen except for the negative control group.Epidermal growth factor was injected subcutaneously once per day for intervention.Eight days later,the body mass,food intake,efficiency of food utilization were observed and the morphology of the intestinal mucous membrane was checked. MAIN OUTCOME MEASURES:①The effect of different doses of epidermal growth factor on the increased body mass,food intake and food intake daily and efficiency of food utilization of the rats daily.②The results of the observation of the intestinal mucous membrane under the optic microscope and the electric-microscope. RESULTS:①There was no significant difference in body mass between the group of rats with epidermal growth factor of 30,20,10 μg/kg and the negative control group as well as positive group(P >0.05)②The daily food intake of the female and male rats in the group with epidermal growth factor of 30,20,10 μg/kg was significantly different compared with that of the ra

关 键 词：表皮生长因子 放射性损伤 保护作用 新疆医科大学第一附属医院 量效关系 Wistar大鼠 食物摄入量 公共卫生学院 阳性对照组 食物利用率 体质量增长 抑制胃酸分泌 肠道损伤 不同剂量 随机对照实验 上皮细胞增生 形态结构 

分 类 号：R735.2[医药卫生—肿瘤] R818.74[医药卫生—临床医学]