CD147对成纤维细胞MMP-9的产生和恶性黑色素瘤细胞浸润的作用  

Effects of CD147 on the production of matrix metalloproteinase-9 by fibroblasts and the invasion of melanoma cells

作　　者：陈翔[1] 田立红[1] 谢红付[1] 施为[1] 冯浩[1] 李吉[1] 陈服文[1] 

机构地区：[1]中南大学湘雅医院皮肤科,长沙410008

出　　处：《中南大学学报（医学版）》2005年第3期249-252,共4页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30200248);湖南省自然科学基金(02jjy4018);湖南省高校学科带头人培养对象(2002);教育部留学回国人员科研启动基金(2003);教育部优秀青年教师资助计划(2003);霍英东青年教师基金(2004);湘雅医院百万人才战略工程基金

摘　　要：目的:探讨CD147分子对成纤维细胞中金属基质蛋白酶9(MMP9)产生和恶性黑色素瘤细胞浸润的可能作用。方法:将表达CD147的恶性黑色素瘤细胞与成纤维细胞共同培养,通过酶谱法检测成纤维细胞中MMP9的表达;并运用体外浸润模型观察恶性黑色素瘤细胞在不同实验条件下的浸润能力。结果:共同培养实验发现,随着黑色素瘤细胞数的增加,培养上清液中MMP9的表达量逐渐增加,CD147抗体可抑制成纤维细胞产生MMP9。当将成纤维细胞放入浸润模型下室底部培养时,黑色素瘤细胞的浸润数量显著增加;在浸润模型上室中加入CD147抗体可显著抑制黑色素瘤细胞的重组基底膜浸润。结论:表达于黑色素瘤细胞的CD147分子可通过刺激成纤维细胞产生MMP9进而在黑色素瘤细胞浸润中发挥作用。Objective To investigate a possible role of CD147 in the production of matrix metalloproteinase-9 (MMP-9) by fibroblasts and the invasion of melanoma cells. Methods We cocultured CD147-expression melanoma cells with fibroblasts and examined the MMP-9 expression of fibroblasts by zymography and the invasion of melanoma cells by transwell invasion assay. Results MMP-9 expression was enhanced in conditioned media, while cocultured with melanoma cells in a dose-dependent manner, and CD147 antibody inhibited the production of MMP-9 in the fibroblasts. When fibroblasts were cultured at the bottom of the lower compartment of transwell invasion model, the number of melanoma cells that invaded significantly increased. Addition of anti-CD147 antibody to the upper compartment transwell invasion model resulted in the significant inhibition of the melanoma cell invasion in reconstituted basement membrane. Conclusion CD147 expressed in melanoma cells plays an important role in the melanoma cell invasion by stimulating the production of MMP-9 by fibroblasts.

关 键 词：CD147 恶性黑色素瘤 浸润 金属蛋白酶-9 

分 类 号：R73-3[医药卫生—肿瘤]