地西泮和莫达非尼对小鼠试验性急性肝衰竭的影响  

Effect of diazepam and modafinil on acute hepatic failure in mice

作　　者：朱海鹏[1] 谭德明[1] 彭仕芳[1] 

机构地区：[1]中南大学湘雅医学院湘雅医院感染病科,长沙410008

出　　处：《中南大学学报（医学版）》2005年第3期344-348,共5页Journal of Central South University ：Medical Science

摘　　要：目的:研究地西泮、莫达非尼对小鼠试验性急性肝衰竭的影响。方法:雄性昆明小鼠240只,给予D半乳糖胺(DGalN)和脂多糖(LPS)同时腹腔注射,制备小鼠急性肝衰竭模型;并于造模前2h,治疗组分别给予相应药物灌胃,对照组给予蒸馏水灌胃。比较各组小鼠的24h存活率,血清转氨酶水平,肝脏组织病理学改变,血清TNFα,IL1的水平,肝脏组织中SOD,MDA,GR,1GSH,NO,NOS的水平。结果:地西泮可以提高小鼠存活率,减轻肝脏病变程度,降低血清转氨酶活性及TNFα,IL1的浓度,增加肝脏组织中SOD,GR的活性,降低NOS的活性及MDA,NO的浓度。莫达非尼可加重肝脏病变程度,升高血清转氨酶的活性及TNFα,IL1的浓度,降低肝脏组织中SOD,GR的活性及升高MDA的浓度。结论:地西泮可减轻DGalN/LPS所致的急性肝衰竭,提高小鼠的存活率,莫达非尼可加重小鼠的肝损伤。Objective To investigate the effect of diazepam and modafinil on acute hepatic failure in mice. Methods Acute liver failure was induced in male Kunming strain mice by enterocoelia injecting the mice with D-GalN and LPS . The mice in the treatment groups were given corresponding drug 2h before the administration of D-GalN and LPS, and the mice in the control group were given the same dose of distilled water. The 24-hour survival rate, serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels were compared. Serum levels of TNF-α and IL-1 and the levels of SOD, MDA, GR, GSH, NO and NOS in the liver were determined. Results Treatment with diazepam increased the survival rate and improved liver histological feature. Diazepam inhibited the serum levels of ALT, AST, TNF-α and IL-1, and reduced levels of MDA, NO and NOS and increased levels of GR and SOD in the liver. Modafinil decreased liver histological feature, increased the serum levels of ALT, AST, TNF-α and IL-1, increased level of MDA, and inhabited levels of SOD and GR in the liver. Conclusion Treatment with diazepam may suppress the D-GalN/LPS-induced acute hepatic failure and modafinil may facilitate the acute hepatic failure.

关 键 词：地西泮 莫达非尼 肝衰竭 

分 类 号：R575.3[医药卫生—消化系统]