14-3-3ζ抑制肺癌细胞转移的实验研究  

Inhibition of metastasis of lung cancer cells by 14-3-3ζ protein:an experimental investigation

作　　者：陈惠华[1] 王妍[1] 徐元基[1] 杜芝燕[1] 郝好杰[1] 徐丁尧[1] 谭晓刚[1] 蔺会云[1] 陆应麟[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《军事医学科学院院刊》2005年第3期227-229,235,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目(30271452);国家"973"计划资助项目(2002CB513105)

摘　　要：目的:探讨14-3-3ζ与肺癌转移的关系。方法:以人肺巨细胞癌高低转移细胞株为模型,Western印迹验证14-3-3ζ在高低转移株中的差异表达;随后采用细胞转染技术将1433ζ基因导入人肺巨细胞癌高低转移株,通过研究转染后细胞增殖能力、黏附能力以及迁移能力的变化,探讨14-3-3ζ与肿瘤转移的相关性。结果:Western印迹检测发现14-3-3ζ在低转移株PLA801C中的表达水平显著高于高转移株PLA801D,在此基础上,构建成功正反义表达载体并转染细胞,转染正义载体后细胞的增殖减慢,黏附能力提高,体外迁移能力下降;而转染反义载体后细胞的增殖加快,体外迁移能力提高。结论:1433ζ可能抑制肺癌细胞的转移。Objective: To investigate the relation between 14-3-3ζ and lung cancer metastasis. Methods:The differential expression of 14-3-3ζ between highly(PLA-801D) and poorly(PLA-801C) metastatic sublines of human lung giant cell carcinoma was examined by Western blot. The sense and antisense expressed vectors of 14-3-3ζ were constructed and transfected into PLA-801C and PLA-801D with Lipofectamine2000. Cell′s ability of proliferation, adhesion and migration after transfection was studied. Results: Western blot exmination found that 14-3-3ζ expressed much more highly in PLA-801C than in PLA-801D. After transfected with the sense expression vector, cells displayed lower proliferation ability, higher adhesion ability and lower migration ability as compared with cells transfected with mock control; while after transfected with the antisense expression vector, cells showed higher proliferation ability and migration ability. Conclusion: 14-3-3ζ might inhibit the metastasis of lung cancer cells.

关 键 词：14-3-3ξ 肿瘤转移 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]