端粒酶反义RNA影响人肝癌细胞系HepG2生长的初步报告  

Primary study on effects of telomerase antisense RNA on human HCC HepG2 cells

作　　者：张东[1] 李开宗[1] 窦科峰[1] 高志清[1] 宋振顺[1] 赵青川[1] 付由池[1] 岳树强[1] 

机构地区：[1]第四军医大学西京医院肝胆外科,西安市710032

出　　处：《中华肝胆外科杂志》2005年第6期417-419,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨抗端粒酶在肝细胞癌治疗中的意义。方法将反义hTR真核表达载体经脂质体介导转染人肝癌细胞系HepG2,体外培养及接种裸鼠观察其基因转染细胞的细胞周期、超微结构变化及致瘤性。结果形态学观察,转染后HepG2细胞出现典型的凋亡现象。FCM检测发现G1期前出现凋亡峰,凋亡率为4·2%。在裸鼠皮下的致瘤性明显降低。HepG2/pBBS212细胞的瘤体抑制率为2·4%,与HepG2/pBBS212-hTR细胞的25·6%相比,差异有显著性(P<0·05)。结论转染端粒酶反义RNA能抑制肝癌HepG2细胞的恶性表型,促进其凋亡。Objective To investigate the role of telomerase antisense RNA in treatment of HCC. Methods HCC HepG2 cells were transfected with antisense hTR expression vector (pBBS-hTR) by lipofection mediation. The gene-transfected cells were cultured in vitro and then inoculated into nude mice to determine the cell cycle, ultrastructure and carcinogenesis of the transfected cells. Results Morphologically, some of the transfected cells manifested typical apoptotic features. FCM showed that the apoptotic peak value appeared in the early phase of cell cycle G1 and the apoptotic rate was 4.2%. The tumorigenesis of transfected cells in nude mice was significantly reduced as compared with that of the controls. The rate of tumor restraining was 2.4% in HepG2/pBBS212 cells and 25.6% in HepG2/pBBS212-hTR cells, respectively(P<0.05). Conclusions The transfection of telomerase antisense RNA can effectively inhibit the growth and promote apoptosis of HCC HepG2 cells.

关 键 词：人肝癌细胞系HEPG2 端粒酶反义RNA HepG2细胞 初步 生长 真核表达载体 基因转染细胞 超微结构变化 脂质体介导 反义HTR 形态学观察 FCM检测 抗端粒酶 细胞周期 体外培养 TR细胞 恶性表型 致瘤性 癌治疗 肝细胞 G1期 凋亡率 

分 类 号：R735.7[医药卫生—肿瘤] R730.21[医药卫生—临床医学]