屈洛昔芬抑制小鼠脑微血管内皮细胞bEnd.3增殖和诱导凋亡的作用  

Effects of growth inhibition and apoptosis induction of droloxifene on mice brain microvascular endothelial cells bEnd.3

作　　者：梁欣[1] 程能能[1] 陈斌艳[1] 王永铭[1] 夏鹏[2] 陈瑛[2] 

机构地区：[1]复旦大学药学院药理学教研室,上海200032 [2]复旦大学药学院药物化学教研室,上海200032

出　　处：《中国临床药学杂志》2005年第3期170-172,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的研究屈洛昔芬对血管内皮细胞的生长抑制和凋亡诱导作用。方法用MTT法测定屈洛昔芬对小鼠脑微血管内皮细胞bEnd.3的增殖抑制率。用Hoechst33258荧光染色及Tunel法检测屈洛昔芬诱导细胞凋亡的作用。结果屈洛昔芬显著抑制bEnd.3细胞增殖,且呈浓度和时间依赖性。12、24、36、48、60、72h的IC50分别为20.31、16.91、12.36、10.66、9.22、8.72μmol·L-1。Hoechst33258荧光染色及Tunel法检测均可见典型的凋亡阳性细胞。结论屈洛昔芬体外可抑制血管内皮细胞生长,且该抑制作用至少部分与诱导细胞凋亡有关。AIM To study the effects of growth inhibition and apoptosis induction of droloxifene on vascular endothelial cells. METHODS Growth inhibition of mice brain microvascular endothelial cells bEnd.3 by droloxifene was analyzed with MTT assay. Droloxifene-induced apoptosis was detected using staining with Hoechst 33258, and further confirmed with Tunel assay. RESULTS Growth of bEnd.3 cells was significantly inhibited by droloxifene in a dose-and time-dependent manner, and the IC 50 for 12,24,36,48,60 and 72 h exposure were 20.31,16.91,12.36,10.66,9.22,8.72 μmol·L -1 respectively. Typical apoptotic cells were detected by Hoechst 33258 staining and Tunel assay. CONCLUSION Droloxifene inhibits the growth of vascular endothelial cells, at least partly due to the induction of apoptosis.

关 键 词：屈洛昔芬 血管内皮细胞 凋亡 

分 类 号：R96[医药卫生—药理学]