幽门螺杆菌对胃癌hMLH1和hMSH2基因表达的影响  

Effect of Helicobacter pylori on hMLH1 and hMSH2 expression in gastric cancer

作　　者：范凯[1] 马坚妹[2] 王朝晖[1] 刘敏[1] 吕申[1] 许国旺[3] 

机构地区：[1]大连医科大学附属二院实验中心,大连116027 [2]大连医科大学解剖教研室,大连116027 [3]中国科学院大连化学物理研究所,大连116023

出　　处：《中国肿瘤临床与康复》2005年第3期202-204,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：中国科学院知识创新工程领域前沿基金资助(No.DICPkaaaabc)

摘　　要：目的探讨幽门螺杆菌(Hp)对胃癌中hMLH1和hMSH2基因表达的影响和Hp的致癌机制。方法利用免疫组织化学SP法检测胃癌、癌旁和胃炎黏膜中hMLH1、hMSH2基因的表达情况。结果在全部被检组织中,Hp感染组hMLH1和hMSH2表达阳性率均低于相应的非感染组,其中胃癌组织中hMSH2表达阳性率在Hp感染组(54.7%)和非感染组(82.1%)差异有显著性(P<0.05)。结论Hp感染引起hMLH1和hMSH2基因表达降低,这可能是Hp导致胃癌的分子机制之一。Objective To investigate the effect of Helicobacter pylori (Hp) on hMLH1 and hMSH2 expression and its possible mechanism of carcinogenesis in gastric cancer.Methods Immunohistochemistry S-P method was used to examine the expression of hMLH1and hMSH2 in cancer tissue, adjacent mucosa and gastritic mucosa.Results In all tissues tested, the positive rates of hMLH1 and hMSH2 expression in Hp infection groups were lower than those in corresponding tissues without Hp infection.The positive rate of hMSH2 expression in cancer tissue with Hp infection(54.7%) was significantly lower than that(82.1%) in tissue without Hp infection(~P<0.05 ).Conclusion Down-regulation of hMLH1 and hMSH2 expression by Hp infection may be one of the molecular mechanisms of gastric cancer resulting from Hp infection.

关 键 词：幽门螺杆菌 胃肿瘤 HMLH1 HMSH2 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]