纤维素酶降解纤维素的最佳酸度和最佳温度的微量量热法研究  

Study of the Optimum Acidity and Temperature of Cellulose Degradation by Cellulase by Microcalorimetry

作　　者：李金花[1] 程远征[1] 张洪林[1] 于淼 

机构地区：[1]曲阜师范大学化学科学学院,山东省曲阜市273165 [2]文登高等职业学校,山东省文登市264400

出　　处：《曲阜师范大学学报（自然科学版）》2005年第3期83-85,共3页Journal of Qufu Normal University(Natural Science)

摘　　要：用微量热法测定了不同酸度和不同温度下纤维素酶降解纤维素的热功率—时间曲线,应用热动力学理论和对比进度法解析出反应的米氏常数(Km)和最大速率(Vmax),并得出速率常数k2,建立速率常数与酸度和温度间的关系式,从而获得纤维素酶降解纤维素的最佳酸度和最佳温度.The power-time curves of cellulose degradation by cellulase at different acidity and different temperature were determined with titration microcalorimeter (Sweden). Using thermokinetic theory and reduced extent method, Km , V max and k2 were calculated. The relationship between k2 and pH, T were constructed. The optimum acidity and optimum temperature of cellulose degradation by cellulase were obtained.

关 键 词：微量热法 热动力学 对比进度 纤维素酶 最佳酸度 最佳温度 

分 类 号：O642.3[理学—物理化学]