人晶状体上皮细胞HGF与c-Met的表达对增殖和凋亡的影响  

Expression of HGF and c-Met in lens epithelial cells and influence on proliferation and apoptosis

作　　者：安小玲[1] 张劲松[1] 韩清[2] 陶津华 

机构地区：[1]中国医科大学附属第一医院眼科,中国辽宁省沈阳市110001 [2]哈尔滨医科大学附属第四医院眼科,中国黑龙江省哈尔滨市150001

出　　处：《国际眼科杂志》2005年第3期445-448,共4页International Eye Science

摘　　要：目的:探讨肝细胞生长因子(hepatocytegrowthfac-tor,HGF)在人晶状体上皮细胞(lensepitheliaclel,lLEC)的表达及其对晶状体上皮细胞的促增殖和抑制凋亡的作用。方法:取原代培养人晶状体上皮细胞,应用RT-PCR,Westernblot检测HGF,c-Met的mRNA及蛋白的表达,应用MTT法检测HGF对晶状体上皮细胞增殖的影响,Western-blo检t测Bcl-2的蛋白表达,以揭示其对晶状体上皮细胞凋亡的影响。结果:HGF和c-Met在人晶状体上皮细胞有表达,HGF有促进晶状体上皮细胞增殖的作用,并能抑制凋亡产生。结论:HGF参与人晶状体上皮细胞的增殖代谢过程,与晶状体上皮细胞的增殖相关。· AIM: To explore the expression of hepatocyte growth factor (HGF) and c-Met in lens epithelial cells (LECs), and to evaluate their roles in proliferation and apoptosis. · METHODS: RT-PCR and Western blot assays were applied to observe the expression of HGF and c-Met's mRNA and protein in LECs in vitro. MTT assay was applied to observe the proliferation of LECs, and Western blot method was used to probe the apoptosis of LECs by the change of Bcl-2.· RESULTS: The HGF and c-Met were expressed in LECs. The LECs proliferated most efficiently under the effect of HGF when the dose is 50μg/L in 24h. The Bcl-2 rose significantly in 1h. · CONCLUSION: HGF and c-Met were expressed in the LECs, and promote their proliferation but inhibit their apoptosis.·

关 键 词：晶状体 上皮细胞 HGF C-MET 基因表达 细胞增殖 细胞凋亡 LEC 肝细胞生长因子 

分 类 号：R776.1[医药卫生—眼科]