高效液相色谱法同时测定头痛灵胶囊中5组分的含量  

Simultaneous determination of caffeine, asipirin, propranolol hydrochloride, prednisone acetate and indometacin in the Toutongling capsules by HPLC

作　　者：奚炜[1] 邢翔飞[1] 熊长友[1] 金桂兰[1] 彭官良[1] 

机构地区：[1]宜昌市第一人民医院,湖北宜昌443000

出　　处：《中国医院药学杂志》2005年第6期508-510,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:采用高效液相色谱法同时测定头痛灵胶囊中阿司匹林、咖啡因、普奈洛尔、醋酸泼尼松和吲哚美辛的含量。方法:采用C18色谱柱,流动相为乙腈0.01mol·L-1磷酸二氢钾(1%三乙胺,用磷酸调节pH2.8)(5050),检测波长为270nm,流速0.9mL·min-1。结果:咖啡因在15.46～48.46mg·L-1,阿司匹林在99.88～299.64mg·L-1,普奈洛尔在5.26～15.78mg·L-1,醋酸泼尼松在4.03～12.09mg·L-1,吲哚美辛在10.04～30.10mg·L-1浓度范围内峰面积与浓度线性关系良好。结论:该方法可同时测定头痛灵胶囊中阿司匹林、咖啡因、普奈洛尔、醋酸泼尼松和吲哚美辛的含量。OBJECTIVETo established a method for simultaneous determination of caffeine, asipirin, propranolol hydrochloride, prednisone acetate and indometacin in the Toutongling capsules by HPLC. METHODSCaffeine, asipirin,propranolol hydrochloride, prednisone acetate and indometacin were eluted with CH_(3)CN: (0.01) mol·L^(-1)KH_(2)PO_(4 )(1% triethyiamine, pH (2.8)) ( 50∶50) on C_(18) column and detected at 270 nm. RESULTSThe Calibration curves were linear in the range of (15.46)-(48.46) mg[DK]·L^(-1)(caffeine),(99.88)-(299.64) mg·L^(-1)(aspirin), (5.26)-(15.78) mg·L^(-1)(propranolol hydrochloride), (4.03)-(12.09) mg·L^(-1 )(prednisone acetate), (10.04)-(30.10) mg·L^(-1)(indometacin). CONCLUSIONThis method is accurate for simultaneous determination of the five components in the capsules.

关 键 词：高效液相色谱法 咖啡因 阿司匹林 普奈洛尔 醋酸泼尼松 吲哚美辛 

分 类 号：R927[医药卫生—药学]