针刺影响下丘脑性肥胖大鼠脂代谢相关因素的分析  

Analysis on the Relevant Factors Involving the Effect of Acupuncture in Improving Lipo-metabolism in Hypothalamic Obesity Rats

作　　者：王少锦[1] 康锁彬[2] 李爱英[3] 佘延芬[1] 赵志国[1] 郑丰杰[1] 

机构地区：[1]河北医科大学中医学院,石家庄050091 [2]河北省中医院,石家庄050011 [3]河北医科大学基础医学课部,石家庄050017

出　　处：《针刺研究》2005年第2期75-79,共5页Acupuncture Research

基　　金：河北省中医药管理局科技攻关项目(No.2003048);河北医科大学中青年科研基金项目(No.030020)

摘　　要：目的:通过针刺肥胖大鼠并作相关实验分析,探讨针刺减肥效应的某些机理。方法:将实验大鼠分为正常组、模型组、针刺组、西布曲明组,观察各组大鼠体重指数(Lee’s指数)、左肾周脂肪重量和脂肪细胞体积、脂质代谢、胰岛素、瘦素及神经肽Y的变化,分析其相互作用与联系。结果:针刺组与模型组相比体重指数、脂质代谢有明显改善(P<0.01),左肾周脂肪湿重降低,脂肪细胞体积减小,与西布曲明组比较针刺组作用更好(P<0.05);针刺组与模型组比较血清胰岛素、瘦素水平明显降低(P<0.01),西布曲明组与模型组比较血清胰岛素、瘦素水平也趋降低(P<0.05);针刺组与模型组比较瘦素与神经肽Y之间有协调趋向,但分布并不一致,西布曲明组则无明显变化。结论:针刺具有减小体重指数、改变脂肪细胞体积、调节脂代谢功能的作用,且可能通过协调机体胰岛素、瘦素及神经肽Y分泌水平而达到降脂减肥目的。Objective: To study the mechanism of acupuncture in reducing body weight in hypothalamic obesity rats. Methods: 80 SD rats were randomly and evenly divided into normal control, model, acupuncture and medication groups. Obesity model was established by subcutaneous injection of 15% sodium glutamate solution (0.2 mL/10 g), once daily and continuously for 5 days. Lee’s index, body weight and length, serum total cholesterol (TCh), triglyceride (TG), high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) were detected with routine biochemical techniques, and serum insulin, leptin and neuropeptide Y contents assayed with radioimmunoassay. EA (dense-sparse waves, frequency of 100 Hz, a suitable strength inducing local muscular tremor) was applied to bilateral “Zusanli” (ST 36)-“Sanyinjiao” (SP 6), and “Guanyuan” (CV 4)-“Zhongwan”(CV 12) for 15 min, once daily and continuously for 28 days. Rats of medication group were fed with Sibutramine (4 mg/kg/d, 28 days altogether). Results: Compared with normal control group, Lee’s index, wet weight of the left perirenal adipose tissue, the area of the lipocytes, serum TG, TCh and LDL-Ch, insulin, leptin and NPY contents and NYP content of the hypothalamus tissue of model group increased significantly (P<0.01); whereas, lipocyte number, serum HDL-Ch and lipoprotein lipase (LPL) and hypothalamic leptin contents of model group decreased significantly (P< 0.01). In comparison with model group, body weight, Lee’s index, wet weight of the left perirenal adipose tissue, area of the lipocytes, serum TG, TCh and LDL-Ch, insulin, leptin and NPY and hypothalamic NPY contents of acupuncture and medication groups lowered considerably (P<0.05～0.01), while the number of lipocytes, serum HDL-Ch and lipoprotein lipase (LPL) and hypothalamic leptin contents of acupuncture and medication groups increased significantly (P<0.05～ 0.01). Comparison between acupuncture and medication groups showed that Lee’s index, serum TG, insulin, lepti

关 键 词：肥胖症 针刺 脂代谢 胰岛素 瘦素 神经肽Y 

分 类 号：R364.2[医药卫生—病理学]