红藻真江篱质粒的发现  

PLASMID FROM THE RED ALGA GRACILARIA ASIATICA OF QINGDAO COAST IN CHINA

作　　者：秦松[1] 童顺 崔武[2] 王希华[1] 李亚非[3] 吴光耀[2] 张培军[1] 曾呈奎[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071 [2]北京大学,北京100871 [3]中国人民解放军肝病研究所,北京100700

出　　处：《海洋与湖沼》1994年第4期349-352,T002,共5页Oceanologia Et Limnologia Sinica

基　　金：中科院海洋研究所所长基金

摘　　要：于1992年4月一1993年4月，用液氮研磨-苯酚／氯仿抽提-异丙醇沉淀的方法提取青岛沿海产紫菜、真江蓠、龙须菜、海膜4种红藻的总DNA；利用Hoechst－CsCl密度梯度起离心法将叶绿体DNA与核DNA分开。对抽取的叶绿体DNA梯度组分电泳结果表明，真江蓠样品出现质粒的电泳条带。以透射电镜观察到闭会环状DNA图像（周长平均值为4.3×10－7m，约为1．3kb）；分子杂交结果显示，真江蓠质位与其叶绿体DNA之间没有同源性，而与核DNA之间有一定同源性。DNAs of the rhodophyte, Gracilaria asiatica, Gracilariopsis lemaneiformis,Porphyra sp. and Halymenia sp. from coast of Qingdao were characterized using Hoechst 33258-Cesium chloride ultracentrifugation from 1992 to 1993. only in G.asiatica, an A-T rich plasmid was co-isolated while it co-migrated with chloroplast DNA in Hoechst-CsCl gradient. Circular and closed pattern was observed under an electron microscope. The girth was 4.3 X10-7m on average, and its size was ca.1300bp. Both gel electrophresis and hybridization showed that its copy number was high. It has homologous sequence with nuclear DNA, instead of chloroplast DNA,which suggest that it may have integrated form.

关 键 词：红藻 真江蓠 质粒 CsCl密度离心 

分 类 号：Q949.29[生物学—植物学]