奥利亚罗非鱼DMO cDNA的分离和克隆  

Cloning DMO cDNA of Oreochromis aurea

作　　者：唐永凯[1,2] 俞菊华[1] 夏德全[1] 王光花 李建林[1] 吴婷婷[1] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心 [2]南京农业大学无锡渔业学院,江苏无锡214081

出　　处：《水产学报》2005年第3期300-306,共7页Journal of Fisheries of China

基　　金：国家"863"项目(2001AA243061);国家自然科学基金(30371116);无锡市自然科学基金(CK030001)

摘　　要：采用RTPCR和RACE法分离和测定了奥利亚罗非鱼DMOcDNA的全序列。得到1571bp[不含poly(A)]的全长cDNA,包括148bp5’非翻译区,1230bp阅读框以及含Poly(A)信号AATAAA的193bp3’非翻译区[不包括Poly(A)]。阅读框共编码409氨基酸,与尼罗罗非鱼DMO编码的氨基酸序列进行比较,同源性为96.3%,表明DMO在同一物种中差别较小。而与尼罗罗非鱼,红鳍东方豚,虹鳟,青鱼将,鼠,人等动物的DMRT1编码的氨基酸序列进行比较,同源性分别为:25.7%,25.8%,24.3%,29.7%,22.5%,22.0%,这说明DMO和DMRT1可能是两个不同的基因。RT-PCR and RACE(rapid amplification cDNA ends ) were used for the isolation of the full length cDNA of DMO gene from ovary of Oreochromis aurea. Sequence analysis revealed a 1571 bp cDNA containing the 148 bp 5'-untranslated region, 193 bp 3'-untranslated region and 1230 bp open reading frame encoding 409 amino acid. Sequence analysis revealed the identity rate of deduced amino acid of DMO in O. aurea and O.niloticus is 96.3%, which showed high homology in species. However, we compared the alignment of deduced amino acid sequences between DMO cDNA from O.aurea and DMRT1 cDNA from O.niloticus, fugu, rainbow trout, medaka, rat and human. The score was 25.7%, 25.8%, 24.3%, 29.7%, 22.5% and 22.0%,respectively. These results indicated that DMO and DMRT1 gene may be different genes.

关 键 词：快速扩增CDNA末端 DMRT1 DMO 奥利亚罗非鱼 

分 类 号：S917[农业科学—水产科学]