高效液相制备色谱制备一枝蒿酮酸对照品  

Preparation of rupestonic acid reference substance by preparative HPLC

作　　者：马雁鸣[1] 赵芸[1] 吴韬[1] 斯拉甫 廖立新[1] 阿吉艾克拜尔.艾萨 

机构地区：[1]中国科学院新疆理化技术研究所 [2]维吾尔医药研究所,新疆乌鲁木齐830001

出　　处：《中草药》2005年第7期1002-1004,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的研究高效液相色谱制备一枝蒿酮酸对照品的方法.方法原料用95%乙醇提取,浓缩成浸膏后用醋酸乙酯萃取.醋酸乙酯部位用100～200目硅胶柱色谱分离,用石油醚-醋酸乙酯(3:1)梯度洗脱,收集样品进行HPLC分离制备.结果该法所得产品用HPLC归一化法定量,质量分数大于98%.结论本方法简便,产品质量高,一枝蒿酮酸产品可用做分析方法的对照品.Objective To establish a preparative method for rupestonic acid reference substance from Artemisia rupestris by HPLC.Methods After raw material being extracted with 95% ethanol,the ethanol extracts were evaporated to dryness under reduced pressure.Then the dry powder was extracted by ethyl acetate,the ethyl acetate extract was fractionated by column chromatography on silica gel(100—200 mesh) using petroleumbenzine-ethyl acetate(3∶1) as the eluent.The collected fraction was purified by preparative HPLC.Results The purity of the product quantitated by normality was over 98%.Conclusion The developed method is simple and rapid and the product of rupestonic acid can be used as reference substance.

关 键 词：新疆一枝蒿 一枝蒿酮酸 对照品 制备高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]