卡介苗(BCG)多克隆抗体的免疫组化染色技术在麻风菌检测中的应用(附120例麻风病检测报告)  

The Role of BCG Polyclonal Antibody in Leprosy Test——A Report of 120 Cases with Leprosy

作　　者：刁兴 潘慧清[1] 何桥[2] 马瑞琼[3] 黄进梅[1] 

机构地区：[1]广东省皮肤性病防治中心,广东广州510500 [2]广东省广州市胸科医院,广东广州510000 [3]广东省广州市皮肤病防治所,广东广州510000

出　　处：《岭南皮肤性病科杂志》2005年第2期105-106,116,共3页Southern China Journal of Dermato-Venereology

摘　　要：目的讨论卡介苗(BCG)多克隆抗体对麻风病的早期诊断,治疗效果评估和复发病人发现的作用。方法收集120例完成MDT、复发、少菌麻风病例。采用免疫组化技术BCG多克隆抗体检测标本组织中麻风杆菌抗原并与10%高碘酸氧化后做抗酸染色和一般抗酸染色做对比。结果BCG组阳性率比10%高碘酸氧化后抗酸染色和一般抗酸染色高。结论BCG多克隆抗体免疫组化染色对麻风病的早期诊断、治疗效果评估、复发的及时发现是一项操作简单而且敏感性高,是较有价值的检测项目。Objective:To explore the role of BCG polyclonal antibody for early diagnosis of leprosy, assessment of therapeutic efficacy, and detection of the recurrent cases. Methods:120 cases of full course of treatment, recurrent and with little germ were collected, and BCG polyclonal antigen of them were tested by immunohistochemistry technology-leprosy polyclonal antibody test compared to coloration by 1% periodate and coloration by common antacid. Results:The positive rate of BCG group was higher than 10% periodate group and the common antacid coloration group. Conclusion:The test of BCG polyclonal antibody was better than coloration of antacid, therefore it is a simple, high sensitivity and with high value test for early leprosy diagnoses, assessment of therapeutic efficacy and promptly detection of the recurrent case.

关 键 词：麻风病 皮肤组织 BCG多克隆抗体 免疫组化 氧化 

分 类 号：R755[医药卫生—皮肤病学与性病学]