黄鳝β-actin基因的克隆及其在鱼类中的系统发生分析(英文)  

Characterization of the β-actin Gene of the Rice Field Eel and Its Phylogeny in Fish

作　　者：夏来新[1] 程汉华[1] 郭一清[1] 周荣家[1] 

机构地区：[1]武汉大学生命科学学院发育生物学研究中心,武汉大学430072

出　　处：《Acta Genetica Sinica》2005年第7期689-695,共7页

基　　金：国家自然科学基金(编号:30270717,30370771);国家重点基础研究发展计划(编号:2004CB117400)资助项目~~

摘　　要：βactin是actin家族的一员,在维持细胞结构,细胞内运动,细胞分裂等细胞生理活动方面发挥着重要的作用。克隆的黄鳝βactin基因的cDNA全长1860bp,编码375个氨基酸,在脊椎动物中不同物种的βactin基因之间的序列同源性超过了98%。RTPCR表明克隆的黄鳝βactin基因在睾丸、卵巢、心、肝、脾、脑等组织中广谱表达。基于目前已知的全部鱼类βactincds,构建了进化树。星形辐射的树型结构一致支持将鱼类βactin基因划分为4类。到目前为止,所有的鱼都没有发现拥有全部4个βactin基因。这暗示伴随着鱼类的辐射式进化历程,可能发生了种系特异性的βactin的丢失。β-actin is a member of the actin family of genes,which play important roles in maintaining cytoskeletal structure,cell motility,cell division,intracellular movements and contractile processes.We report here the identification of a β-actin cDNA of the rice field eel,a teleost fish with a characteristic of natural sex reversal.The cDNA sequence of this gene was 1 860 bp in length,encoding a 375 amino acid protein.Amino acid identities of the β-actin between the rice field eel and other vertebrates including human,chicken,and other fishes,were more than 98%.RT-PCR showed expression of the rice field eel β-actin in testis,ovotestis,ovary,heart,liver,spleen and brain,suggesting a ubiquitous expression pattern.Phylogenetic tree including all β-actin cds of teleost fishes was constructed,which suggested consistently that teleost β-actin can be classified into four types,but no fish has been found contains all the four types of β-actin gene in its genome.The results that suggest lineage-specific β-actin loss might happen in the radical evolution of teleost fishes.

关 键 词：Β-ACTIN 黄鳝 性反转 系统发生 

分 类 号：Q78[生物学—分子生物学]