二乙基亚硝胺诱发新生大鼠肝癌的实验模型  

AN EXPERIMENTAL MODEL ON DIETHYLNITROSAMINE HEPATOCARCINOGENESIS IN NEWBORN WISTAR RATS

作　　者：周光兴[1] 杨俊华[1] 赵为之[1] 彭松林[1] 

机构地区：[1]上海医科大学实验动物部

出　　处：《上海医科大学学报》1994年第2期97-100,T007,共5页Journal of Fudan University(Medical Science)

摘　　要：用肝化学致癌物二乙基亚硝胺（ＤＥＮ）按照Ｐｅｒａｉｎｏ诱癌模式建立ＤＥＮ大鼠肝：癌模型，并对ＤＥＨ作用时间与次数加以改进，多阶段观察大鼠肝脏癌前期病变、肿瘤发生率及６种酶组织化学染色。结果表明本实验诱癌过程短，敏感性高，方法简单；碱性磷酸酶（ＡＫＰ）、琥珀酸脱氢酶（ＳＤＨ）及羧酸酯酶作为肝癌酶标记具有潜在的应用价值。提示以新生大鼠肝细胞活跃增生为基础的Ｐｅｒａｉｎｏ诱发肝癌模式，改进ＤＢＮ的处理方式，对于大鼠癌前期病变和肿瘤发生有较大的影响。涉及的影响因素可能与大鼠性别及ＤＥＮ的双重效应有关。In this study,newborn male and female rats that one day after birth eath of the offspring were injected once ip with diethylnitrosamine(DEN) in 0.9% NaCl solution at a dosage of 0.15μmol/g body weight.The one half of the total number of rats received repeat ip injection of DEN at a dosage of 0.15μmol/g body weight beginning at weanling, after the rats were weaned onto a 30% casein diet containning 0.05% phenobarbital.The rats were killed at intervals and their livers were examined for preneoplastic lesions and tumor.Six histochemical markers were used to detect foci of altered hepatocyts.The results indicate that alkaline phosphatase,succinate dehydrogenase and carboxylesterase appeared as potential markers for neoplastic nodules and tumorigenesis detection,the sex of the Wistar rats and injection time of DEN had influence on the incidences of tumors.

关 键 词：二乙基亚硝胺 癌前病变 肝肿瘤 

分 类 号：R735.702[医药卫生—肿瘤]