香蕉束顶病毒复制酶和黄瓜花叶病毒衣壳蛋白融合基因植物表达载体的构建  

Construction of plant expression vector of fusion genes with Banana bunchy top virus replicase and Cucumber mosaic virus coat protein

作　　者：郑耘[1] 李华平[1] 肖火根[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室,广东广州510642

出　　处：《华南农业大学学报》2005年第3期18-21,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金资助项目(39870434);广东省科技攻关项目(C20209)

摘　　要：利用重组PCR技术将香蕉束顶病毒的复制酶基因和黄瓜花叶病毒的衣壳蛋白基因融合重组,并与植物表达载体pBI121连接,构建了抗香蕉束顶病和香蕉花叶病的双价植物表达载体,通过冻融法转化载体入根癌农杆菌LBA4404,获得了工程农杆菌pBI121.FBC,从而为培育抗病转基因香蕉品种奠定了基础.Two genes, the replicase gene from Banana bunchy top virus and coat protein gene from Cucumber mosaic virus, were firstly fused together with PCR, and were inserted into a plant expression vector-pBI121. The plant expression vector was transformed to Agrobacterium tumefaciens LBA4404. As a result, the engineered agrobacteria containing pBI121.FBC were gotten, and would be further used to develop a engineering banana variety resistant to two kinds of viruses.

关 键 词：香蕉束顶病毒 黄瓜花叶病毒 融合基因 

分 类 号：S432.4[农业科学—植物病理学]