肝纤维化大鼠骨代谢及其调控激素的改变  

Study on the Changes of Bone Metabolism in CCl_4-Induced Liver Fibrosis Rats

作　　者：俞华芳[1] 朱金水[2] 余小虎[2] 

机构地区：[1]复旦大学附属金山医院消化内科,上海200540 [2]交通大学附属第六人民医院消化内科,上海200233

出　　处：《中国临床医学》2005年第3期435-437,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:研究肝纤维化大鼠骨代谢及其调控激素的改变。方法:20只大鼠分为2组,一组以四氯化碳肌注建立肝纤维化大鼠模型,另一组为正常对照组。分别测定2组的肝功能[丙氨酸氨基转移酶(alanineaminotmnsferase,ALT)、天门冬氨酸氨基转移酶(as—partameaminotransferase,AST)、总胆红素(totalbilirubin,TB)、总蛋白(serumtotalprotein,TP)、白蛋Lh/球蛋白(A/G)、γ谷氨酰转移酶(γ-glutamyltransferase,γ-GT)];股骨和腰椎骨密度(bonemineraldensity,BMD);骨代谢指标及其调控激素[血清钙、尿钙、25羟基维生素D3[25(OH)VitD3]、甲状旁腺激素(parathyroidhormone,PTH)、尿吡啶酚/肌酐(urinarypyridinoline/ereatinine,Pyd/Cr)]。结果:肝纤维化大鼠与正常对照组相比,股骨和腰椎骨密度下降,但无显著差异[0.149±0.014vs0.151±0.006g/cm2,P>0.05;0.139±0.012vs0.146±0.007g/cm2,P>0.05];血清25(OH)VitD3无显著差异[18.97±1.52vs18.20±1.03ng/ml,P>0.05];尿Pyd/Cr则显著升高[0.13±0.04vs0.08±0.02nm/μmol,P<0.01]。相关性分析显示,腰椎骨密度与血清A/G呈正相关(r=0.586,P<0.01),而与尿Pyd/Cr呈负相关(r=-0.512,P<0.05)。结论:肝纤维化大鼠骨吸收加强。Objective: To investigate the bone metabolism of CCl4-induced liver fibrosis rats. Methods: Twenty SD rats were divided into 2 groups, liver fibrosis rats induced by CCLi group (n = 10) and control group (n = 10). The liver function parameters[aspartame amin-otransferase(AST), alanine aminotransferase(ALT), γ-glutamyltransferase (γ-GT),serum total protem(TP),albumin(ALB),total biliru-bin(TB)],bone mineral density(BMD)of lumbar vertebra and femoral bone and bone metabolic parameters and its modulating hormones [ parathyroid hormone (PTH).serum calcium,25-OH vitamin D3,urinary pyridinoline/creatinine(Pyd/Cr)]were measured. Results: Compared with the controls, the ESMD in lumbar vertebra and femoral bone of cirrhosis rats decreased, but there were no statistically significane (0.149 ± 0. 014vs 0. 151 ± 0. 006 g/cm2, P>0. 05) 0. 139 ± 0. 012vs 0. 146 ± 0.007 g/cm2 , P>0. 05). Serum 25(OH) VitD3 in the liver fibrosis group was not significantly lower than that of the controls(18. 97 ± 1. 52 vs 18. 20 ± 1. 03ng/ml, P>0. 05) .while urinary Pyd/Cr of rats with liver fibrosis was significantly higher than that of the control group(0. 13 ± 0. 04 vs 0. 08 ± 0. 02 nm/μmol, P<0.01). Conclusion: Bone resorption increases in liver fibrosis rats induced by CCl4.

关 键 词：肝纤维化 骨代谢 肝功能 骨密度 大鼠 

分 类 号：R575.29[医药卫生—消化系统]