鼻咽及其邻近部位各类型癌组织中EBV　DNA的原位检测  

In situ detection of EBV DNA in various types of carcinomas derived from the nasopbarynx and neighboring regions

作　　者：蒋晓群[1] 姚开泰[1] 

机构地区：[1]长沙湖南医科大学肿瘤研究所

出　　处：《中华病理学杂志》1994年第2期85-88,T021,共5页Chinese Journal of Pathology

基　　金：美国中华医学基金会(CMB;NewYork);国家八五计划;湖南省卫生厅资助课题

摘　　要：改进了生物素标记探针与石蜡组织切片的ＤＮＡ－ＤＮＡ原位杂交技术，采用ＥＢ病毒的ＢａｍＨＩ－Ｗ片段为探针，检测鼻咽部及其邻近部位各种不同组织学和分化类型的癌组织及慢性炎症标本中ＥＢＶ的存在与分布情况。结果表明：ＥＢ病毒的存在与癌的组织类型和分化程度密切相关，并不局限于鼻咽部。在鼻咽部的低、中、高分化鳞状细胞癌标本，ＥＢＶＤＮＡ阳性例数分别为３９／４１个、１／７个和０／３个；胯部及扁桃体低鳞癌标本的阳性例数分别为３／４个和２／９个；少部分鼻咽低分化腺癌（２／８个）标本中也发现ＥＢＶＤＮＡ；头颈部其它部位的中、高分化鳞癌及良性肿瘤中未见ＥＢＶＤＮＡ的存在。在阳性标本，ＥＢＶＤＮＡ存在于几乎所有的癌细胞胞核中，而标本的间质细胞、浸润的淋巴细胞、癌旁上皮细胞和慢性鼻咽炎症的上皮细胞为阴性。sensitive and reliable technique for in situ hybridization of DNA-DNA with biotin labelled probe on routine paraffin sections was developed. Using BanJH I-W fragment of EBV as probe, a variety of biopsies from nasopharynx and its neighboring regions were examined for EBV distribution.It was dis-covered that the existance of E BV was related to the histological type and degree of differentiation of carcinomas,but not limited to the nasopharynx region. The EBV DNA positive ratios in(1)poorly,(2)moderately and(3) well differentiated nasopharyngeal squamous cell carcinomas,(4) poorlv differenti-ated nasopharyngeal adenocarcinoma,(5) poorly differentiated squamous cell carcinomas derived from palate and(6)tonsil were 39 /41,1/7,0/3,2/8,3/4 and 2/9 respectively.No EBV DNA was de-tected in other moderately and well differentiated squamous cell carcinomas or benign tumors ofthe head and neck EBV DNA was detccted in nearly all maligiiant cell nuclei of positive sections,whereas all morphologically normal and hyperplastic epithelial cells, stroma cells,infiltrating lymphocytes and epithelium in chronic nasopharyngitis were negative.

关 键 词：鼻咽肿瘤 头颈部肿瘤 杂交 E-B病毒 

分 类 号：R739.630.3[医药卫生—肿瘤]