人卵巢癌细胞系HO－8910的建立及其生物学特性  

The establishment of human ovarian carcinoma cell line HO-8910 and its characteristics.

作　　者：牟瀚舟[1] 许沈华[1] 张奕荫 钱丽娟[1] 黄晓曙 朱赤红[1] 高永良[1] 

机构地区：[1]浙江省肿瘤研究所

出　　处：《中华妇产科杂志》1994年第3期162-164,T013,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：以分化差的卵巢乳头状浆液性囊腺癌患者腹水为材料，建立了体外培养的人卵巢癌细胞系ＨＯ－８９１０。细胞已传代１３０次，生长良好；组织学和超微结构均显示该细胞具有恶性上皮细胞特征；染色体数目分布在４５～３００之间，众数为５４（４２％），流式细胞仪检测ＤＮＡ指数为１．４５，均呈超二倍体；该细胞能分泌性激素，并有激素受体存在；裸鼠移植瘤与患者原始肿瘤病理形态基本一致；细胞经液氮冻存后复苏良好。该细胞系的建立为卵巢癌防治研究提供了体外实验材料。cell line designated HO-8910 was establishedin vitro from the ascitic fluid of a patient withpoorly-differentiated ovarian papillary serious cyst-adenocarcinoma. The cell line grew well through 130passages and showed malignant epithelial morphologyunder light and electron microscopes. Thechromosome number varied widely from 45 to 300 andits model was 54(42%);The DNA index was 1.45 asmeasured by FCM method;Both of them showed aneuploidy.The cell line has the ability to secretesteroid sex honnones and also has hormone receptors. When transplanted into nude mice, the xenograft hassimilar pathological characteristic to the original. It isexpected that this cell line would be of great help in thefuture study on ovarian carcinoma.

关 键 词：细胞株 卵巢肿瘤 细胞分化 HO-8910 

分 类 号：R737.310.3[医药卫生—肿瘤]