能量可控陡脉冲对荷瘤大鼠免疫功能的影响  

Experimental Study of Energy Controllable Steep Pulse in the Treatment of Rat with Subcutaoneous Transplantive Tumor

作　　者：罗小东[1] 胡丽娜[1] 孙才新[2] 姚陈果[2] 熊兰[2] 米彦[2] 王士彬[2] 

机构地区：[1]重庆医科大学第二附属医院妇产科,重庆400010 [2]重庆大学高电压与电工新技术教育部重点实验室,重庆400030

出　　处：《重庆大学学报（自然科学版）》2005年第7期106-109,共4页Journal of Chongqing University

基　　金：国家自然科学基金资助项目(30271484)

摘　　要：采用细胞悬液注射法建立Wistar大鼠皮下瘤模型,研究能量可控陡脉冲(ECSP)对荷瘤大鼠免疫功能的影响.ECSP处理后利用MTT法检测各组大鼠脾淋巴细胞转化率及NK细胞活性,ELISA法检测大鼠腹腔巨噬细胞分泌TNF-α的表达.结果表明ECSP处理组肿瘤生长明显受到抑制;ECSP处理组淋巴细胞转化率、NK细胞活性及TNF-α的表达明显高于荷瘤对照组(P<0.05,P<0.05,P<0.05),且与正常对照组相似(P=0.953,P=0.130,P=0.080).ECSP可以抑制肿瘤生长,诱发机体抗癌免疫反应,改善和恢复荷瘤鼠淋巴细胞和巨噬细胞的免疫活性.To investigate the effects of immune function of energy controllable steep pulse on the transplanted tumor in rat. Tumor size were measured before and after treatment to evaluate the reaction of the treatment. After two weeks of treatment, MTT methods were used to observe the lymphocytes proliferation and the natural killer cells(NK) activity. TNF-α activities was measured by ELISA. The result shows that the growth of the tumor is significantly inhibited in ECSP therapy group as compared with the controls. It is founded that the Lymphocytes proliferation, the natural killer cells(NK) activity and TNF-α activities in ECSP therapy group is similar to that of the normal control group, there is no obvious difference(P=0.953, P=0.130, P=0.080),but these parameters are markedly higherly than the that of the control group (P<0.05,P<0.05,P<0.05). ECSP can inhibit the growth of the tumor and induce the host immune reaction of anti-tumor efficiently and improve the immune activity of lymphocyte and macrophage in rats with tumor.

关 键 词：能量可控陡脉冲 免疫效应 肿瘤 

分 类 号：R730.5[医药卫生—肿瘤]