人成骨肉瘤细胞系（OS－732）对骨质破坏吸收的病理  

Destruction and Resorption of Neonatal BonySpicules by a Human Osteosarcoma Cell Line:ALight and Ultrastructural Study

作　　者：刘子君[1] 丘钜世[1] 文剑明[1] 张萌[1] 梁凤英 陶瑜[1] 徐栋梁[1] 

机构地区：[1]中山医科大学病理教研室

出　　处：《中华骨科杂志》1994年第11期672-675,T002,共5页Chinese Journal of Orthopaedics

摘　　要：用人成骨肉瘤ＯＳ－７３２细胞系与人胎骨粒共培养，观察瘤细胞浸润及溶解吸收骨质的过程。可见（１）瘤细胞突侵入骨质浅层，包绕骨基质致呈小块状分离，然后吸收；（２）瘤细胞分泌水解酶，直接溶解及吸收骨基质，致骨质表层陷窝状凹陷。故认为瘤细胞能直接浸润及溶解骨质，而不需要破骨细胞的参与。作者尚应用￣（４５）Ｃａ标记新生小鼠颅骨与人成骨肉瘤细胞系共培养，以及加入骨质溶解促进剂（ＥＧＦ）及抑制剂（二磷酸盐）后，通过液闪计数仪检测以及￣（４５）Ｃａ释出的数据，加以比较及分析。结果说明ＥＧＦ能使骨质溶解增强；小剂量二磷酸盐可抑制骨质的溶解，但大剂量二磷酸盐则有加速骨肉瘤细胞溶解骨质的作用。AbstractInvasion and resorption of human neonatalbony spicules bv coculture with cells of osteosar-coma cell line(0S一732 )were studied and themorphology observed by light and electron micro-scopes。The process of invasion、and resorption ofbonv matrix by tumor cells can be summarized intotwo patterns。(1)Cytoplasmic processes or mi-crovill of tumor cells invaded and eroded the su-perficial layers of bony matrix into small pieces,followed by phagocytosis and resorption of tumorcells。(2 ) Secretion of lysosomal enzymes,possi-bly collagenase or some other lytic factors by tu-mor cells causing direct lysis and resorption of lo-cal bony matrix with formation ofHowship's lacu-na where the tumor cells were located. All thesemorphological changes indicated that invasion andresorption of bone were mainly by osteosarcomacells l and no evidence of participation by osteo-clasts during the whole course of these process. Inthis report the liberation of ￣(45)Ca labelled in neona-tal mouse calvaria into the medium after coculturewith the osteosarcoma cells were assaved by scin-tillation,and in some specimens were superaddedwith EGF or diphosphonates at the same times inorder to measure their enects on the liberation of￣(45)Ca。Theresults showed that theosteosarcomacells can increase the liberation of ￣(45)Ca into themedium as compared with the control group with-out addition of tumor cells。 Addition of EGF totumor cells can stimulate the increased release of￣(45)Ca , while small dose of diphosphanates showedevidence of suppressing effects, On the contrary,when larger dose of diphosphonates were used,enhancing effect on the liberation of ￣(45)Ca into themedium was shown .

关 键 词：肉瘤 细胞系 骨质破坏 钙离子释放 骨肿瘤 

分 类 号：R738.102[医药卫生—肿瘤]