肝癌组织IGF－II、IGF－IIR、HBxAg的表达与细胞DNA倍体和S期的关系  

A STUDY OF THE RELATIONSHIP BETWEEN EXPRESSION OF IGF-II、IGF-IIR,HBxAg AND THE DNA PLOIDY,CELL CYCLE OF HEPATOCYTES IN HEPATOCRCINOMA

作　　者：邓涛[1] 陈乃玲[1] 贾克明[1] 

机构地区：[1]中国人民解放军肝病研究所

出　　处：《中华内科杂志》1994年第11期743-746,T021,共4页Chinese Journal of Internal Medicine

摘　　要：　为了探讨胰岛素样生长因子ＩＩ（ＩＧＦ－ＩＩ）在肝癌发生发展中的作用，采用免疫组织化学ＰＡＰ法检测了肝癌组织ＩＧＦ－ＩＩ及其受体（ＩＧＦ－ＩＩＲ）、乙型肝炎病毒Ｘ抗原（ＨＢｘＡｇ）的表达，并用流式细胞术分析了ＩＧＦ－ＩＩ表达与细胞ＤＮＡ倍体、Ｓ期的关系。结果显示：（１）ＩＧＦ－ＩＩ、ＩＧＦ－ＩＩＲ、ＨＢｘＡｇ在血清乙型肝炎病毒（ＨＢＶ）标记阳性的癌组织阳性率均为９３％（ｎ＝１５），高于ＨＢＶ标记阴性的癌组织（１／５）（Ｐ＜０．０５）；（２）ＩＧＦ－ＩＩ阳性的癌组织ＤＮＡ异倍体出现率为１００％、Ｓ期比例为２８．８±６．４％，高于ＩＧＦ－ＩＩ阴性的癌组织ＤＮＡ异倍体出现率（６０％）和Ｓ期比例（１２．８±２．４％）（Ｐ＜０．０５）；（３）肝癌组织ＩＧＦ－ＩＩ、ＩＧＦ－ＩＩＲ的表达与ＨＢｘＡｇ表达一致。研究结果提示ＩＧＦ－ＩＩ可能在乙型肝炎病毒标志物（ＨＢＶ－Ｍ）阳性的肝细胞癌发生发展中有某种作用，ＨＢｘＡｇ可能是ＩＧＦ－ＩＩ基因的一种激活因子。n order to study the role of insulin-like growth factor II ( IGF-II)in the development of hepatocarci-noma(HCC),the expression of IGF-II,IGF-Il receptor(IGF-IIR)and HBxAg in HCC was studied with immunohistochemistry(PAP method).Meanwhile DNA ploidy and S-phase fraction of hepatocytes were analyzed with flow cytometry.The results were as follows:(1) IGF-II,IGF-IIR and HBxAg showed posi-tive staining simultaneouesly in the tumor tissues of 93%(n=15) of the HCC cases with chronic liver disease and with positive evidence of HBV;(2)The mean S-phase incidence in tissues of IGF-II positive HCC was 28.6±6.4%;this was higher than l2. 8± 2.4%in the IGF-Il negative tumors(P<0. 05);(3)The incidence of DNA-aneuploidy in IGF-II positive liver tissues was 100%(10110);this was higher han 60%(6/10) in IGF-II negative liver tissues (P<0.05). It is suggested that IGF-II might play an important role in the development of HCC when there is evidence of HBV and chronic liver disease involve-ment.IGF-II positive staining HCC have increased proliferative activity as compared with IGF-II negative staining tumors.

关 键 词：肝肿瘤 肝细胞癌 胰岛素样 生长因子II DNA 

分 类 号：R735.703[医药卫生—肿瘤]