肝细胞癌变过程中p53的突变和甲胎蛋白的表达  

Mutation of p53 gene and expression of alpha-fetoprotein during hepatocarcinogenesis

作　　者：邹合强[1] 汤钊猷[1] 叶胜龙[1] 余业勤[1] 马曾辰[1] 周信达[1] 刘康达[1] 鲍卫华[1] 

机构地区：[1]上海医科大学肝癌研究所

出　　处：《中华医学杂志》1994年第8期474-475,T047,共3页National Medical Journal of China

摘　　要：对４例正常人肝、５例肝硬变、５例肝腺瘤样增生（ＡＨ）、１６例肝细胞癌（ＨＣＣ）（Ｅｄｍｏｎｄｓｏｎ分级Ｉ、ＩＩ、ＩＩＩ级各４、６、６例）组织中突变型ｐ５３蛋白（ｍＰ５３）和甲胎蛋白（ＡＦＰ）进行了免疫组化检测。结果发现：正常人肝ｍＰ５３、ＡＦＰ均阴性。５例肝硬变组织中，４例同时显示ｍＰ５３和ＡＦＰ阳性，１例显示二者皆阴性。５例ＡＨ中，４例为ｍＰ５３、ＡＦＰ阳性，１例为二者阴性。在１６例ＨＣＣ中，４例１级者，２例显示ｍＰ５３、ＡＦＰ均阳性，２例为二者均阴性；６例ＩＩ级者，４例ｍＰ５３和ＡＦＰ均阳性，２例二者均阴性；６例ＩＩＩ级者，１例ｍＰ５３阳性ＡＦＰ阴性，２例ｍＰ５３阴性ＡＦＰ阳性，３例二者均阴性。结果提示：ｐ５３的突变可能发生在肝细胞癌变过程的早期，与癌变过程的启动有关；其作用可能与ＡＦＰ的重新激活有关。o investigate the expression of mutant p53 pro-tein (mP53) and alpha-fetoprotein (AFP) duringhepatocarcinogenesis, we detected immunohistoch-emically the specimens from 4 cases of normal hu-man liver. 5 of cirrhosis, 5 of adenomatoushyperplasia (AH), and 16 of hepatocellularcareinoma (HCC) (by Edmondson classification. The4 cases with normal liver showed negative mP53 andAFP. Four of the 5 cirrhosis cases were positive formP53 and AFP. In 5 AH cases, 4 were positive formP53 and AFP. In the 4 cases of grade I HCC, 2were positive for mP53 and AFP. In the 6 cases ofgrade II HCC, 4 were positive for mP53 and AFP. Inthe 6 cases of grade III HCC, 1 showed mP53positive staining but negative AFP, and 2 were nega-tive for mP53 but positive for AFP, while 3 were neg-ative for both mP53 and AFP. The results indicatedthat the mutation of p53 gene occurred in the earlystage of hepatocarcinogenesis, and may be correlatedwith the initiation of hepatocarcinogenesis, and thatmutant p53 protein probably related to thereactivation of AFP gene.

关 键 词：基因 P53 甲胎蛋白 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]